• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过夹心酶联免疫吸附测定法对人类III型嗜T细胞病毒/淋巴结病相关病毒及获得性免疫缺陷综合征相关逆转录病毒样临床分离株进行检测与鉴别。

Detection and differentiation by sandwich enzyme-linked immunosorbent assay of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus- and acquired immunodeficiency syndrome-associated retroviruslike clinical isolates.

作者信息

Higgins J R, Pedersen N C, Carlson J R

出版信息

J Clin Microbiol. 1986 Sep;24(3):424-30. doi: 10.1128/jcm.24.3.424-430.1986.

DOI:10.1128/jcm.24.3.424-430.1986
PMID:2428827
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268927/
Abstract

Monoclonal antibodies can be used in sandwich enzyme-linked immunosorbent assays to measure viral antigens. Such an assay was developed to detect the core protein, p24, of human T-cell lymphotropic virus type III and lymphadenopathy-associated virus, etiologic agents of the acquired immunodeficiency syndrome (AIDS). Another AIDS-associated virus, AIDS-associated retrovirus type 2 (ARV-2) could not be detected in this assay because of the low affinity of one of the monoclonal antibodies to ARV-2 p24. Detection of ARV-2 was accomplished with a monoclonal antibody-rabbit polyclonal antibody sandwich enzyme-linked immunosorbent assay. These two assays were used to efficiently detect AIDS-related viruses in lymphocyte cell cultures and to distinguish strains of the viruses.

摘要

单克隆抗体可用于夹心酶联免疫吸附测定以检测病毒抗原。已开发出这样一种测定法来检测人类Ⅲ型嗜T细胞病毒和淋巴结病相关病毒的核心蛋白p24，这两种病毒是获得性免疫缺陷综合征（艾滋病）的病原体。另一种与艾滋病相关的病毒，2型艾滋病相关逆转录病毒（ARV-2），在该测定法中无法检测到，因为其中一种单克隆抗体与ARV-2 p24的亲和力较低。采用单克隆抗体-兔多克隆抗体夹心酶联免疫吸附测定法完成了对ARV-2的检测。这两种测定法被用于有效检测淋巴细胞细胞培养物中的艾滋病相关病毒，并区分病毒株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/8860a9528571/jcm00099-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/7e8c33184fd9/jcm00099-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/1ab470f2a129/jcm00099-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/8860a9528571/jcm00099-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/7e8c33184fd9/jcm00099-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/1ab470f2a129/jcm00099-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc8f/268927/8860a9528571/jcm00099-0130-a.jpg

相似文献

1
Detection and differentiation by sandwich enzyme-linked immunosorbent assay of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus- and acquired immunodeficiency syndrome-associated retroviruslike clinical isolates.通过夹心酶联免疫吸附测定法对人类III型嗜T细胞病毒/淋巴结病相关病毒及获得性免疫缺陷综合征相关逆转录病毒样临床分离株进行检测与鉴别。
J Clin Microbiol. 1986 Sep;24(3):424-30. doi: 10.1128/jcm.24.3.424-430.1986.
2
[Isolation of human immunodeficiency virus from Hungarian AIDS and ARC patients].[从匈牙利艾滋病和艾滋病相关综合征患者中分离出人类免疫缺陷病毒]
Orv Hetil. 1990 Apr 29;131(17):895-8.
3
Persistent infection of chimpanzees with human immunodeficiency virus: serological responses and properties of reisolated viruses.黑猩猩持续感染人类免疫缺陷病毒：血清学反应及重新分离病毒的特性
J Virol. 1987 Oct;61(10):3173-80. doi: 10.1128/JVI.61.10.3173-3180.1987.
4
Unique p24 epitope marker to identify multiple human immunodeficiency virus variants in blood from the same individuals.用于鉴定同一人血液中多种人类免疫缺陷病毒变体的独特p24表位标志物。
J Clin Microbiol. 1987 Aug;25(8):1411-5. doi: 10.1128/jcm.25.8.1411-1415.1987.
5
Detection of human T-cell lymphotropic virus type III-related antigens and anti-human T-cell lymphotropic virus type III antibodies by anticomplementary immunofluorescence.用抗补体免疫荧光法检测人III型嗜T细胞病毒相关抗原及抗人III型嗜T细胞病毒抗体
J Clin Microbiol. 1986 Jun;23(6):1072-7. doi: 10.1128/jcm.23.6.1072-1077.1986.
6
Correlation of enzyme-linked immunosorbent assays for serum human immunodeficiency virus antigen and antibodies to recombinant viral proteins with subsequent clinical outcomes in a cohort of asymptomatic homosexual men.血清人类免疫缺陷病毒抗原及针对重组病毒蛋白的抗体的酶联免疫吸附测定与一组无症状同性恋男性后续临床结局的相关性
Am J Med. 1987 Aug;83(2):208-12. doi: 10.1016/0002-9343(87)90686-3.
7
Pathogenesis of HIV and its implications for serodiagnosis and monitoring of antiviral therapy.HIV的发病机制及其在血清学诊断和抗病毒治疗监测中的意义。
J Virol Methods. 1987 Aug;17(1-2):19-34. doi: 10.1016/0166-0934(87)90065-6.
8
Lymphadenopathy-associated virus type 2 in AIDS and AIDS-related complex. Clinical and virological features in four patients.艾滋病及艾滋病相关综合征中的2型淋巴结病相关病毒。4例患者的临床和病毒学特征
Lancet. 1987 Jan 17;1(8525):128-32. doi: 10.1016/s0140-6736(87)91967-2.
9
Isolation of a lymphadenopathy-associated virus from a patient with the acquired immune deficiency syndrome.
S Afr Med J. 1985 Aug 3;68(3):144-7.
10
Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection.艾滋病、艾滋病相关综合征及亚临床人类免疫缺陷病毒感染中针对核心抗原和包膜抗原的抗原血症及抗体滴度
J Infect Dis. 1987 Mar;155(3):558-60. doi: 10.1093/infdis/155.3.558.

引用本文的文献

1
A rapid label-free method for quantitation of human immunodeficiency virus type-1 particles by nanospectroscopy.纳米光谱法快速定量检测人类免疫缺陷病毒 1 型颗粒。
J Virol Methods. 2012 Jun;182(1-2):70-5. doi: 10.1016/j.jviromet.2012.03.012. Epub 2012 Mar 20.
2
Direct inactivation of human immunodeficiency virus type 1 by a novel small-molecule entry inhibitor, DCM205.新型小分子进入抑制剂DCM205对1型人类免疫缺陷病毒的直接灭活作用
Antimicrob Agents Chemother. 2007 May;51(5):1780-6. doi: 10.1128/AAC.01001-06. Epub 2007 Feb 16.
3
Efavirenz therapy in rhesus macaques infected with a chimera of simian immunodeficiency virus containing reverse transcriptase from human immunodeficiency virus type 1.

本文引用的文献

1
Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.用于研究通过凝胶电泳分离的抗原和抗体特异性的酶联免疫电转移印迹技术（EITB）。
Methods Enzymol. 1983;92:377-91. doi: 10.1016/0076-6879(83)92032-3.
2
Purification and characterization of the RNA-directed DNA polymerase of a primate type-D retrovirus: Mason-Pfizer virus.灵长类D型逆转录病毒——梅森- Pfizer病毒RNA指导的DNA聚合酶的纯化及特性分析
Biochim Biophys Acta. 1980 May 30;607(3):445-56. doi: 10.1016/0005-2787(80)90155-0.
3
Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes.
用依法韦仑治疗感染了含有1型人类免疫缺陷病毒逆转录酶的猿猴免疫缺陷病毒嵌合体的恒河猴。
Antimicrob Agents Chemother. 2004 Sep;48(9):3483-90. doi: 10.1128/AAC.48.9.3483-3490.2004.
4
Susceptibilities of simian immunodeficiency virus to protease inhibitors.猿猴免疫缺陷病毒对蛋白酶抑制剂的敏感性。
Antimicrob Agents Chemother. 2003 May;47(5):1756-9. doi: 10.1128/AAC.47.5.1756-1759.2003.
5
Self-stabilized antisense oligodeoxynucleotide phosphorothioates: properties and anti-HIV activity.自稳定反义寡脱氧核苷酸硫代磷酸酯：性质与抗HIV活性
Nucleic Acids Res. 1993 Jun 11;21(11):2729-35. doi: 10.1093/nar/21.11.2729.
6
Principles of laboratory isolation and identification of the human immunodeficiency virus (HIV).人类免疫缺陷病毒（HIV）实验室分离与鉴定原则
Yale J Biol Med. 1987 Nov-Dec;60(6):575-87.
7
Release of interleukin 1 inhibitory activity (contra-IL-1) by human monocyte-derived macrophages infected with human immunodeficiency virus in vitro and in vivo.体外和体内感染人类免疫缺陷病毒的人单核细胞衍生巨噬细胞释放白细胞介素1抑制活性（抗白细胞介素1）
J Clin Invest. 1988 Dec;82(6):2097-105. doi: 10.1172/JCI113831.
8
Clinical laboratory applications of monoclonal antibodies.单克隆抗体的临床实验室应用。
Clin Microbiol Rev. 1988 Jul;1(3):313-29. doi: 10.1128/CMR.1.3.313.
9
Rapid and accurate viral diagnosis.快速准确的病毒诊断。
Pharmacol Ther. 1989;40(2):287-328. doi: 10.1016/0163-7258(89)90098-3.
10
Inhibition of human immunodeficiency virus (HIV) replication by HIV-trans-activated alpha 2-interferon.HIV反式激活α2干扰素对人类免疫缺陷病毒（HIV）复制的抑制作用
Proc Natl Acad Sci U S A. 1989 Jul;86(13):4958-62. doi: 10.1073/pnas.86.13.4958.
淋巴结病相关病毒（LAV）对辅助诱导性T淋巴细胞的选择性嗜性。
Science. 1984 Jul 6;225(4657):59-63. doi: 10.1126/science.6328660.
4
Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.皮肤T细胞淋巴瘤体外增殖的丝裂原需求
Blood. 1980 Mar;55(3):409-17.
5
Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.从旧金山艾滋病患者中分离淋巴细胞病变逆转录病毒。
Science. 1984 Aug 24;225(4664):840-2. doi: 10.1126/science.6206563.
6
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.从艾滋病患者和有患艾滋病风险的人群中频繁检测和分离出细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):500-3. doi: 10.1126/science.6200936.
7
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.从艾滋病患者和艾滋病前期患者中检测、分离并持续生产细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):497-500. doi: 10.1126/science.6200935.
8
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).从一名有获得性免疫缺陷综合征（艾滋病）风险的患者体内分离出一种嗜T淋巴细胞逆转录病毒。
Science. 1983 May 20;220(4599):868-71. doi: 10.1126/science.6189183.
9
Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27.
J Immunol Methods. 1983 Jan 28;56(2):209-20. doi: 10.1016/0022-1759(83)90413-1.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.