Croxson S, Mildvan D, Mathews H, Poiesz B J
Department of Medicine, Beth Israel Medical Center, New York, New York 10003.
J Clin Microbiol. 1988 Jul;26(7):1304-8. doi: 10.1128/jcm.26.7.1304-1308.1988.
Replication of human immunodeficiency virus (HIV) may be initiated in infected lymphocytes by antigenic or mitogenic stimulation. A soluble protein derived from an invasive strain of Entamoeba histolytica (amoebic antigen [AA]) was used to study the lymphoblastic responses of T lymphocytes derived from 8 HIV-seronegative homosexual men (controls) and 15 HIV-seropositive homosexual men (patients). The soluble protein was also used in long-term cultures as a stimulus for HIV replication. No control or patient produced detectable lymphoblastic responses to AA in a 6-day tritiated-thymidine incorporation assay. Of 15 patients, 5 (33%) produced HIV p24 (ranging from 31 pg/ml to 151 ng/ml) in response to AA in 30-day cell cultures. HIV p24 was expressed in three of seven patients in response to AA but not to the T-lymphocyte mitogen phytohemagglutinin. Implications for managing HIV-infected patients are discussed.
人类免疫缺陷病毒（HIV）的复制可通过抗原或丝裂原刺激在受感染的淋巴细胞中启动。一种源自溶组织内阿米巴侵袭性菌株的可溶性蛋白（阿米巴抗原[AA]）被用于研究8名HIV血清阴性同性恋男性（对照组）和15名HIV血清阳性同性恋男性（患者）的T淋巴细胞的淋巴细胞反应。该可溶性蛋白也被用于长期培养，作为HIV复制的刺激物。在为期6天的氚化胸腺嘧啶核苷掺入试验中，没有对照组或患者对AA产生可检测到的淋巴细胞反应。在15名患者中，5名（33%）在30天的细胞培养中对AA产生了HIV p24（范围从31 pg/ml至151 ng/ml）。7名患者中有3名对AA产生了HIV p24，但对T淋巴细胞丝裂原植物血凝素没有反应。文中讨论了对管理HIV感染患者的意义。
