Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Physiol Biochem. 2013 Sep;69(3):527-37. doi: 10.1007/s13105-013-0241-z. Epub 2013 Mar 1.
Indomethacin (IND) is a non-steroid anti-inflammatory agent that is known to induce severe gastric mucosal lesions. In this study, we investigated the protective effect of selenium (SEL), grape seed extract (GSE), and both on IND-induced gastric mucosal ulcers in rats. Sprague-Dawley rats (200-250 g) were given SEL, GSE, and both by oral gavage for 28 days, and then gastric ulcers were induced by oral administration of 25 mg/kg IND. Malondialdehyde (MDA), non-enzymatic (reduced glutathione, GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants, prostaglandin E2 (PGE2) in gastric mucosa, and serum tumor necrosis factor alpha (TNF-α) were measured. Moreover, gastric ulcer index and preventive index were determined. Indomethacin increased the gastric ulcer index, MDA, TNF-α, and decreased PGE2 and non-enzymatic (GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants. Pretreatment with SEL, GSE, and both significantly decreased the gastric ulcer index, MDA, and TNF and increased antioxidants and PGE2. Histopathological observations confirm the gastric ulcer index and biochemical parameters. Selenium and GSE have a protective effect against IND-induced gastric ulcers through prevention of lipid peroxidation, increase of GSH, activation of radical scavenging enzymes, PGE2 generation, and anti-inflammatory activity. Co-administration of GSE and SEL is more effective than GSE or SEL alone.
吲哚美辛（IND）是一种非甾体抗炎药，已知会导致严重的胃黏膜损伤。在这项研究中，我们研究了硒（SEL）、葡萄籽提取物（GSE）和两者对大鼠 IND 诱导的胃黏膜溃疡的保护作用。给予 Sprague-Dawley 大鼠（200-250g）口服 SEL、GSE 和两者 28 天，然后口服 25mg/kg IND 诱导胃溃疡。测量胃黏膜丙二醛（MDA）、非酶（还原型谷胱甘肽，GSH）和酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）抗氧化剂、前列腺素 E2（PGE2）和血清肿瘤坏死因子-α（TNF-α）。此外，还测定了胃溃疡指数和预防指数。吲哚美辛增加了胃溃疡指数、MDA、TNF-α，并降低了 PGE2 和非酶（GSH）和酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）抗氧化剂。SEL、GSE 和两者的预处理显著降低了胃溃疡指数、MDA 和 TNF，并增加了抗氧化剂和 PGE2。组织病理学观察证实了胃溃疡指数和生化参数。SEL 和 GSE 通过预防脂质过氧化、增加 GSH、激活自由基清除酶、生成 PGE2 和抗炎活性对 IND 诱导的胃溃疡具有保护作用。GSE 和 SEL 的联合给药比 GSE 或 SEL 单独给药更有效。
