Thornburg L P, Rottinghaus G, McGowan M, Kupka K, Crawford S, Forbes S
College of Veterinary Medicine, Department of Statistics, University of Missouri, Columbia.
Vet Pathol. 1990 Mar;27(2):81-88. doi: 10.1177/030098589002700202.
Histomorphologic, histochemical, and atomic absorption analysis studies were performed on liver tissue from 623 clinically normal purebred and mixed-breed dogs to assess the range of hepatic copper concentrations, the histologic distribution of copper, and the histomorphologic changes associated with the various copper concentrations that were found. Atomic absorption analysis revealed a continuous numerical spectrum ranging from less than 100 parts per million on a dry weight basis (ppm dw) to more than 2,000. No decisive numerical criterion was found that could be used to separate normal from abnormal copper concentrations because of this continuous array. The threshold for histochemical demonstration of copper-containing granules using rhodanine or rubeanic acid staining was 400 ppm dw. At this concentration the copper-containing granules were located in the centrilobular hepatocytes (zone 3 of Rappaport). Copper-containing granules were found in the midzonal and periportal hepatocytes (zones 2 and 1 of Rappaport) in livers with 1,000 ppm dw and higher copper concentrations. The majority of the 623 livers were normal histomorphologically. Multifocal hepatitis characterized by mixed inflammatory cell accumulation and centrilobular distribution was found to be associated with copper concentrations in the range of 2,000 ppm dw or higher. An appropriate upper limit for normal hepatic copper concentration in the dog was not determined based on the present study.
对623只临床正常的纯种和混血犬的肝脏组织进行了组织形态学、组织化学和原子吸收分析研究，以评估肝脏铜浓度范围、铜的组织学分布以及与所发现的各种铜浓度相关的组织形态学变化。原子吸收分析显示，以干重计，连续数值范围从每百万份少于100份（ppm dw）到超过2000份。由于这种连续排列，未发现可用于区分正常与异常铜浓度的决定性数值标准。使用罗丹宁或黑尿酸染色进行含铜颗粒组织化学显示的阈值为400 ppm dw。在此浓度下，含铜颗粒位于小叶中央肝细胞（Rappaport的3区）。在铜浓度为1000 ppm dw及更高的肝脏中，在中间带和门周肝细胞（Rappaport的2区和1区）中发现了含铜颗粒。623只肝脏中的大多数在组织形态学上是正常的。以混合性炎症细胞积聚和小叶中央分布为特征的多灶性肝炎被发现与2000 ppm dw或更高的铜浓度有关。基于本研究，未确定犬肝脏正常铜浓度的合适上限。
