脂质提取与分析。
Lipid Extraction and Analysis.
机构信息
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
出版信息
Methods Mol Biol. 2022;2468:271-281. doi: 10.1007/978-1-0716-2181-3_14.
Abstract
Lipids are major components of cellular membranes and energy stores. Lipids contribute vital structural, energetic, and signaling functions. We have optimized methods to extract and analyze lipids from the nematode Caenorhabditis elegans based on standard methods. Here we describe a method to extract total lipids from C. elegans larvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze fatty acid composition from lipid extracts, lipid fractions, or directly from nematode larvae, adults, or embryos.
摘要
脂质是细胞膜和能量储存的主要成分。脂质具有重要的结构、能量和信号功能。我们基于标准方法优化了从线虫秀丽隐杆线虫中提取和分析脂质的方法。在这里，我们描述了一种从线虫幼虫、成虫或胚胎中提取总脂质的方法。我们描述了一种薄层层析法来分离主要脂质类，以及一种气相色谱法来分析脂质提取物、脂质馏分或直接从线虫幼虫、成虫或胚胎中脂肪酸组成。
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