嗜热噬菌体TP-84的生产与纯化。
Production and purification of the thermophilic bacteriophage TP-84.
作者信息
Epstein I, Campbell L L
出版信息
Appl Microbiol. 1975 Feb;29(2):219-23. doi: 10.1128/am.29.2.219-223.1975.
Abstract
A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58-C were essential for maximal phage production. Phage (5 times 1011 infective particles/ml) was precipitated with polyethylene glycol (molecular weight 6,000) in the presence of sodium chloride and was further purified by cesium chloride density centrifugation.
摘要
描述了一种高产生产和纯化嗜热噬菌体TP - 84的新方法。嗜热脂肪芽孢杆菌菌株10的培养物，用营养物质富集以获得大量生长并防止孢子形成，并保持在pH 6.5，在100升发酵罐中用噬菌体感染。添加氯化镁（0.01 M）和58℃的温度对于最大噬菌体产量至关重要。噬菌体（5×10¹¹感染性颗粒/毫升）在氯化钠存在下用聚乙二醇（分子量6000）沉淀，并通过氯化铯密度离心进一步纯化。
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