噬菌体phiNS11：一种嗜酸性嗜热细菌的含脂噬菌体。II. 噬菌体的纯化及一些特性

Bacteriophage phiNS11: a lipid-containing phage of acidophilic thermophilic bacteria. II. Purification and some properties of the phage.

作者信息

Sakaki Y, Yamada K, Oshima M, Oshima T

出版信息

J Biochem. 1977 Nov;82(5):1451-6. doi: 10.1093/oxfordjournals.jbchem.a131833.

DOI:10.1093/oxfordjournals.jbchem.a131833

Abstract

An improved procedure for the purification of a lipid-containing acidophilic thermophilic bacteriophage, phiNS11, is described. The procedure involves Cs2SO4 discontinuous density gradient, Cs2SO4 equilibrium density gradient and sucrose gradient centrifugations. The phage has an icosahedral shape, 75 nm in diameter, with spike-like structures radiating from the vertices. It contained about 12% by weight of lipid, 13--14% of DNA and 74--75% of protein. The phage was stable between pH 2 and 5, but rapidly inactivated above pH 6. It was highly sensitive to organic solvents and detergents. Some other basic properties such as the buoyant density and UV spectrum of the purified phage are also described.

摘要

本文描述了一种改进的用于纯化含脂质嗜酸性嗜热噬菌体phiNS11的方法。该方法包括硫酸铯不连续密度梯度离心、硫酸铯平衡密度梯度离心和蔗糖梯度离心。该噬菌体呈二十面体形状，直径75纳米，有从顶点辐射出的刺状结构。它含有约12%（重量）的脂质、13 - 14%的DNA和74 - 75%的蛋白质。该噬菌体在pH 2至5之间稳定，但在pH 6以上迅速失活。它对有机溶剂和去污剂高度敏感。还描述了纯化噬菌体的一些其他基本特性，如浮力密度和紫外光谱。