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正常和发育中的油棕花和果实的转录组分析。

Transcriptome analysis of normal and mantled developing oil palm flower and fruit.

机构信息

National Center for Genetic Engineering and Biotechnology, 113 Phahonyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.

出版信息

Genomics. 2013 May;101(5):306-12. doi: 10.1016/j.ygeno.2013.02.012. Epub 2013 Mar 5.

DOI:10.1016/j.ygeno.2013.02.012
PMID:23474141
Abstract

Elaeis guineensis (oil palm) accounts for a large and increasing proportion of the world's cooking oil production. Cloning via somatic embryogenesis results in a somaclonal variant known as mantled which produce fruit with little to no oil yield. The mantled phenotype is believed to be epigenetic in nature. We performed RNA-Seq on developing flower and fruit samples of normal and mantled oil palm to characterize their transcriptomes. We present expression data for all transcripts in normal and mantled flower and fruit samples. Many genes are differentially expressed, including several from pathways that may be the cause of the mantled phenotype if disrupted, such as genes involved in primary hormone responses, DNA replication and repair, chromatin remodeling and a gene involved in RNA mediated DNA methylation. In addition, the gene expression data for developing flower and fruit will serve as a valuable resource for oil palm genetics and genomic studies.

摘要

油棕(Elaeis guineensis)在世界食用油生产中占有很大且不断增加的比例。通过体细胞胚胎发生进行克隆会产生一种称为“mantled”的体细胞变异体,其果实几乎不含油。mantled 表型被认为是表观遗传的。我们对正常和 mantled 油棕发育中的花和果实样本进行了 RNA-Seq 分析,以表征它们的转录组。我们提供了正常和 mantled 花和果实样本中所有转录本的表达数据。许多基因表达存在差异,包括几个可能是 mantled 表型原因的通路中的基因,如参与初级激素反应、DNA 复制和修复、染色质重塑以及一个涉及 RNA 介导的 DNA 甲基化的基因。此外,花和果实发育的基因表达数据将成为油棕遗传和基因组研究的宝贵资源。

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