School of Life and Basic Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, People's Republic of China.
Funct Integr Genomics. 2013 Jun;13(2):241-51. doi: 10.1007/s10142-013-0316-5. Epub 2013 Mar 9.
Plant annexins are Ca(2+)- and phospholipid-binding proteins forming an evolutionary conserved multi-gene family. They are implicated in the regulation of plant growth, development, and stress responses. With the availability of the maize genome sequence information, we identified 12 members of the maize annexin genes. Analysis of protein sequence and gene structure of maize annexins led to their classification into five different orthologous groups. Expression analysis by RT-PCR revealed that these genes are responsive to heavy metals (Ni, Zn, and Cd). The maize annexin genes were also found to be regulated by Ustilago maydis and jasmonic acid. Additionally, the promoter of the maize annexin gene was analyzed for the presence of different stress-responsive cis-elements, such as ABRE, W-box, GCC-box, and G-box. RT-PCR and microarray data show that all 12 maize annexin genes present differential, organ-specific expression patterns in the maize developmental steps. These results indicate that maize annexin genes may play important roles in the adaptation of plants to various environmental stresses.
植物 annexins 是一类 Ca(2+)-和磷脂结合蛋白，形成一个进化上保守的多基因家族。它们参与植物生长、发育和应激反应的调节。随着玉米基因组序列信息的可用，我们鉴定了 12 个玉米 annexin 基因成员。对玉米 annexin 蛋白序列和基因结构的分析将其分为五个不同的直系同源群。通过 RT-PCR 的表达分析表明，这些基因对重金属（Ni、Zn 和 Cd）有反应。还发现玉米 annexin 基因受玉米黑粉菌和茉莉酸的调控。此外，还分析了玉米 annexin 基因启动子中是否存在不同的应激响应顺式元件，如 ABRE、W-box、GCC-box 和 G-box。RT-PCR 和微阵列数据显示，在玉米发育阶段，12 个玉米 annexin 基因均呈现出不同的、器官特异性的表达模式。这些结果表明，玉米 annexin 基因可能在植物适应各种环境胁迫中发挥重要作用。
