Sakalli Erdal, Baylancicek Serdar, Yuksel Meral, Erdurak Selcuk Cem, Dadas Burhan
Sisli Etfal Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey.
Int J Pediatr Otorhinolaryngol. 2013 May;77(5):792-5. doi: 10.1016/j.ijporl.2013.02.013. Epub 2013 Mar 9.
A close relationship between reactive oxygen species (ROS) and myringosclerosis, which is a common complication of myringotomy, was recently reported. The objective of this study was to measure ROS levels directly in rat tympanic membranes using luminol-aided chemiluminescence (CL) in order to compare the levels of ROS after incisional and radiofrequency (RF) myringotomy.
Fifteen Sprague-Dawley rats were separated into three groups of five animals each. Bilateral myringotomies were made using an appropriate myringotomy lancet in Group 1 and RF in Group 2. Group 3 served as the control group with no myringotomy. Twenty-four hours after the procedure, all tympanic membranes were inspected with an otomicroscope and then excised for the measurement of ROS using luminol-aided CL.
The mean ROS level in Group 1 was significantly higher than that in Groups 2 and 3 (p<0.05 for both). The difference in mean ROS level between Groups 2 and 3 was not significant (p>0.05). Otomicroscopy revealed increased vascularity and vessel dilation in all tympanic membranes that underwent myringotomy. Vascular dilation was observed in the annular region in the vessels that passed along the long arm of the malleus, in addition to the vessels feeding the anterior and posterior tympanomalleolar folds.
Although the relationship between ROS and the development of myringosclerosis after myringotomy has been demonstrated, the present study is the first to compare incisional and RF myringotomy based on the measurement of ROS levels. Our results indicate that the increase in ROS due to myringotomy was greater following incisional myringotomy than RF myringotomy.
最近有报道称活性氧（ROS）与鼓膜切开术的常见并发症鼓膜硬化症之间存在密切关系。本研究的目的是使用鲁米诺辅助化学发光（CL）直接测量大鼠鼓膜中的ROS水平，以比较切开式和射频（RF）鼓膜切开术后的ROS水平。
将15只Sprague-Dawley大鼠分为三组，每组五只动物。第1组使用合适的鼓膜切开刀进行双侧鼓膜切开术，第2组使用射频进行。第3组作为未进行鼓膜切开术的对照组。术后24小时，用耳显微镜检查所有鼓膜，然后切除用于使用鲁米诺辅助CL测量ROS。
第1组的平均ROS水平显著高于第2组和第3组（两者p<0.05）。第2组和第3组之间的平均ROS水平差异不显著（p>0.05）。耳显微镜检查显示，所有接受鼓膜切开术的鼓膜血管增多和血管扩张。除了供应鼓膜前后皱襞的血管外，在沿锤骨长臂走行的血管的环形区域也观察到血管扩张。
虽然已经证明了ROS与鼓膜切开术后鼓膜硬化症发展之间的关系，但本研究是首次基于ROS水平的测量比较切开式和RF鼓膜切开术。我们的结果表明，切开式鼓膜切开术后因鼓膜切开术导致的ROS增加比RF鼓膜切开术更大。
