Rozwadowska Beata, Hudzik Grzegorz
Wojewódzka Stacja Sanitarno-Epidemiologiczna w Katowicach.
Przegl Epidemiol. 2012;66(4):587-90.
The aim of this study was to analyze Borrelia burgdorferi genostrains appearing in the Silesian voivodeship in years 2010-2011 and the first half of 2012. MATERIAL AND METHODS. The analysis was conducted on blood samples of persons bitten by ticks and ticks provided to The Provincial Sanitary Epidemiological Station in Katowice from patients. The material was examined under the angle of three genostrains of Borrelia burgdorferi: Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii with using real-time PCR method. RESULTS. It was shown that only two genostrains of Borrelia burgdorferi were detected in the nucleic acid extracts from samples i.e.: Borrelia burgdorferi sensu stricto and Borrelia afzelii. Borrelia garinii wasn't detected in the nucleic acid extracts. CONCLUSIONS. Study on the prevalence of individual Borrelia burgdorferi genostrains are important in assessing the risk of infection spirochetes inhabitants of The Silesian province and persons staying there temporarily.
本研究的目的是分析2010 - 2011年以及2012年上半年出现在西里西亚省的伯氏疏螺旋体基因菌株。材料与方法。分析对象为被蜱虫叮咬者的血液样本以及患者提供给卡托维兹省卫生防疫站的蜱虫。采用实时聚合酶链反应方法,从伯氏疏螺旋体的三种基因菌株角度对材料进行检测:狭义伯氏疏螺旋体、阿氏疏螺旋体和伽氏疏螺旋体。结果。结果显示,在样本的核酸提取物中仅检测到两种伯氏疏螺旋体基因菌株,即:狭义伯氏疏螺旋体和阿氏疏螺旋体。在核酸提取物中未检测到伽氏疏螺旋体。结论。研究单个伯氏疏螺旋体基因菌株的流行情况对于评估西里西亚省居民和临时居住在该地区的人员感染螺旋体的风险具有重要意义。