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Optimization of non-radioactive Southern blot hybridization: single copy detection and reuse of blots.

作者信息

Allefs J J, Salentijn E M, Krens F A, Rouwendal G J

机构信息

Centre for Plant Breeding Research, Wageningen, The Netherlands.

出版信息

Nucleic Acids Res. 1990 May 25;18(10):3099-100. doi: 10.1093/nar/18.10.3099.

DOI:10.1093/nar/18.10.3099
PMID:2349131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330882/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd8/330882/702db8602ceb/nar00194-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd8/330882/6d4edaf2b335/nar00194-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd8/330882/702db8602ceb/nar00194-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd8/330882/6d4edaf2b335/nar00194-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebd8/330882/702db8602ceb/nar00194-0245-a.jpg

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Optimization of non-radioactive Southern blot hybridization: single copy detection and reuse of blots.非放射性Southern印迹杂交的优化：单拷贝检测及印迹的重复使用
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本文引用的文献

1
Alkaline transfer of DNA to plastic membrane.DNA向塑料膜的碱性转移
Biochem Biophys Res Commun. 1984 Jul 18;122(1):340-4. doi: 10.1016/0006-291x(84)90480-7.
2
Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
3
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.DNA和RNA向硝酸纤维素或重氮苄氧基甲基纸的双向转移。
Net1 的 C 端区域是 RNA 聚合酶 I 转录的激活因子，在从酵母到人中具有保守特征。
PLoS Genet. 2019 Feb 25;15(2):e1008006. doi: 10.1371/journal.pgen.1008006. eCollection 2019 Feb.
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Detection of HBV Covalently Closed Circular DNA.乙型肝炎病毒共价闭合环状DNA的检测
Viruses. 2017 Jun 6;9(6):139. doi: 10.3390/v9060139.
5
Different CMS sources found in Beta vulgaris ssp maritima: mitochondrial variability in wild populations revealed by a rapid screening procedure.在滨海甜菜亚种 Beta vulgaris ssp maritima 中发现了不同的 CMS 来源：通过快速筛选程序揭示野生种群中的线粒体变异性。
Theor Appl Genet. 1993 Jan;85(5):529-35. doi: 10.1007/BF00220909.
6
Mitochondrial DNA diversity and male sterility in natural populations of Daucus carota ssp carota.胡萝卜自然种群中线粒体 DNA 多样性与雄性不育性。
Theor Appl Genet. 1995 Jul;91(1):150-9. doi: 10.1007/BF00220872.
7
Abundance and length polymorphism of microsatellite repeats in Beta vulgaris L.菜心微卫星重复序列的丰富度和长度多态性
Theor Appl Genet. 1996 Mar;92(3-4):326-33. doi: 10.1007/BF00223675.
8
Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules.酿酒酵母中活跃转录的rRNA基因被组织在与高迁移率族蛋白Hmo1相关的特殊染色质中，并且基本上没有组蛋白分子。
Genes Dev. 2008 May 1;22(9):1190-204. doi: 10.1101/gad.466908.
9
Fitness of isogenic colony morphology variants of Pseudomonas aeruginosa in murine airway infection.铜绿假单胞菌同基因菌落形态变体在小鼠气道感染中的适应性
PLoS One. 2008 Feb 27;3(2):e1685. doi: 10.1371/journal.pone.0001685.
10
Pathogenesis of type II avian adenovirus infection in turkeys: in vivo immune cell tropism and tissue distribution of the virus.火鸡II型禽腺病毒感染的发病机制：病毒在体内的免疫细胞嗜性和组织分布
J Virol. 1996 Jan;70(1):30-6. doi: 10.1128/JVI.70.1.30-36.1996.
Anal Biochem. 1980 Nov 15;109(1):123-9. doi: 10.1016/0003-2697(80)90019-6.
4
Rapid transfer of DNA from agarose gels to nylon membranes.DNA从琼脂糖凝胶快速转移至尼龙膜。
Nucleic Acids Res. 1985 Oct 25;13(20):7207-21. doi: 10.1093/nar/13.20.7207.
5
A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.乙型肝炎病毒DNA杂交检测中化学发光底物与比色底物的比较。
Anal Biochem. 1989 Jul;180(1):95-8. doi: 10.1016/0003-2697(89)90093-6.
6
Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.DNA的化学发光检测：在DNA测序和杂交中的应用。
Nucleic Acids Res. 1989 Jul 11;17(13):5115-23. doi: 10.1093/nar/17.13.5115.
7
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
8
Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.通过斑点杂交法测定核酸序列同源性和相对浓度。
Nucleic Acids Res. 1979 Nov 24;7(6):1541-52. doi: 10.1093/nar/7.6.1541.