Canonico P G, McManus A T, Mangiafico J A, Sammons L S, McGann V G, Dangerfield H G
Infect Immun. 1975 Mar;11(3):466-9. doi: 10.1128/iai.11.3.466-469.1975.
The burst in oxidative metabolism that is mediated through activation of the hexose monophosphate shunt and accompanies particle ingestion by polymorphonuclear leukocytes was used as the indicator in an in vitro radiometabolic assay for detection of specific opsonizing antibody to Francisella tularensis. Release of 14CO2 from radiolabeled glucose was increased significantly when specific immune serum added to suspensions of monkey polymorphonuclear leukocytes and F. tularensis. With this method, opsonizing antibodies to F. tularensis were detected in monkey serum 3 days after vaccination. Significantly increased opsonic activity in these monkeys preceded the appearance of, and persisted longer than, antibody activity as determined by conventional serological techniques. In addition, sera from 11 of 12 humans that were immunized 1 month to 13 years previously and had nondiagnostic agglutinating antibody titers demonstrated significant opsonizing activity.
通过激活磷酸己糖旁路介导的、伴随多形核白细胞摄取颗粒而出现的氧化代谢爆发，被用作一种体外放射代谢测定中的指标，用于检测针对土拉弗朗西斯菌的特异性调理抗体。当向猴多形核白细胞和土拉弗朗西斯菌的悬液中加入特异性免疫血清时，放射性标记葡萄糖释放的14CO2显著增加。用这种方法，在接种疫苗3天后在猴血清中检测到了针对土拉弗朗西斯菌的调理抗体。这些猴中调理活性的显著增加先于常规血清学技术所测定的抗体活性出现，且持续时间更长。此外，12名在1个月至13年前免疫、凝集抗体效价无诊断意义的人中，有11人的血清表现出显著的调理活性。
