Nakano M, Noguchi T, Sugioka K, Fukuyama H, Sato M
J Biol Chem. 1975 Mar 25;250(6):2404-6.
In the presence of Fe3+-ADP and NADPH, washed rat liver microsomes at 37 degrees and at pH 7.5 emitted ultraweak light with the peroxidative cleavage of endogenous lipid. The main light-emitting species of this system was analyzed spectroscopically in the visible region and estimated to be singlet oxygen. The addition of superoxide dismutase or catalase did not inhibit the light emission significantly. It is unlikely therefore that superoxide anion and OH are involved in the generation of singlet oxygen in the present system.
在Fe3+-ADP和NADPH存在的情况下,洗涤后的大鼠肝脏微粒体在37℃、pH 7.5条件下,内源性脂质发生过氧化裂解并发出超弱光。该系统的主要发光物质在可见光区域进行了光谱分析,估计为单线态氧。添加超氧化物歧化酶或过氧化氢酶并不能显著抑制发光。因此,超氧阴离子和OH不太可能参与本系统中单线态氧的产生。