Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA.
BMC Vet Res. 2013 Mar 8;9:47. doi: 10.1186/1746-6148-9-47.
Despite the importance of abnormalities in lipoprotein metabolism in clinical canine medicine, the fact that most previously used methods for lipoprotein profiling are rather laborious and time-consuming has been a major obstacle to the wide clinical application and use of lipoprotein profiling in this species. The aim of the present study was to assess the feasibility of a continuous lipoprotein density profile (CLPDP) generated within a bismuth sodium ethylenediaminetetraacetic acid (NaBiEDTA) density gradient to characterize and compare the lipoprotein profiles of healthy dogs of various breeds, healthy Miniature Schnauzers, and Miniature Schnauzers with primary hypertriacylglycerolemia. A total of 35 healthy dogs of various breeds with serum triacylglycerol (TAG) and cholesterol concentrations within their respective reference intervals were selected for use as a reference population. Thirty-one Miniature Schnauzers with serum TAG and cholesterol concentrations within their respective reference intervals and 31 Miniature Schnauzers with hypertriacylglyceridemia were also included in the study.
The results suggest that CLPDP using NaBiEDTA provides unique diagnostic information in addition to measurements of serum TAG and cholesterol concentrations and that it is a useful screening method for dogs with suspected lipoprotein metabolism disorders. Using the detailed and continuous density distribution information provided by the CLPDP, important differences in lipoprotein profiles can be detected even among dogs that have serum TAG and cholesterol concentrations within the reference interval. Miniature Schnauzers with serum TAG and cholesterol concentrations within the reference interval had significantly different lipoprotein profiles than dogs of various other breeds. In addition, it was further established that specific lipoprotein fractions are associated with hypertriacylglyceridemia in Miniature Schnauzers.
The results of the present study suggest that density gradient ultracentrifugation using NaBiEDTA is a useful screening method for the study of lipoprotein profiles in dogs. Therefore, this method could potentially be used for diagnostic purposes for the separation of dogs suspected of having lipoprotein abnormalities from healthy dogs.
尽管脂蛋白代谢异常在兽医临床中具有重要意义，但由于大多数先前用于脂蛋白谱分析的方法相当繁琐和耗时，这一直是该方法在该物种中广泛临床应用和使用的主要障碍。本研究旨在评估使用乙二胺四乙酸钠（NaBiEDTA）双折射密度梯度生成连续脂蛋白密度谱（CLPDP）来描述和比较不同品种健康犬、健康迷你雪纳瑞犬和原发性高三酰甘油血症迷你雪纳瑞犬的脂蛋白谱的可行性。选择了 35 只血清三酰甘油（TAG）和胆固醇浓度在各自参考区间内的不同品种的健康犬作为参考人群。还纳入了 31 只血清 TAG 和胆固醇浓度在各自参考区间内的迷你雪纳瑞犬和 31 只高三酰甘油血症迷你雪纳瑞犬。
结果表明，使用 NaBiEDTA 的 CLPDP 除了测量血清 TAG 和胆固醇浓度外，还提供了独特的诊断信息，并且是一种有用的筛查方法，可用于疑似脂蛋白代谢紊乱的犬。使用 CLPDP 提供的详细连续密度分布信息，即使在血清 TAG 和胆固醇浓度在参考区间内的犬中，也可以检测到脂蛋白谱的重要差异。血清 TAG 和胆固醇浓度在参考区间内的迷你雪纳瑞犬的脂蛋白谱与其他不同品种的犬有显著差异。此外，进一步确定特定的脂蛋白亚群与迷你雪纳瑞犬的高三酰甘油血症有关。
本研究的结果表明，使用 NaBiEDTA 的密度梯度超速离心是一种有用的犬脂蛋白谱研究筛查方法。因此，这种方法有可能用于诊断目的，用于将疑似脂蛋白异常的犬与健康犬分离。
