Department of Veterinary Clinical Sciences, University of Minnesota, College of Veterinary Medicine, St. Paul, Minnesota, USA.
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences, Texas A & M University, College Station, Texas, USA.
J Vet Intern Med. 2024 Mar-Apr;38(2):971-979. doi: 10.1111/jvim.17010. Epub 2024 Feb 13.
Hypertriglyceridemia (HTG) is prevalent in Miniature Schnauzers, predisposing them to life-threatening diseases. Varied responses to management strategies suggest the possibility of multiple subtypes.
HYPOTHESIS/OBJECTIVE: To identify and characterize HTG subtypes in Miniature Schnauzers through cluster analysis of lipoprotein profiles. We hypothesize that multiple phenotypes of primary HTG exist in this breed.
Twenty Miniature Schnauzers with normal serum triglyceride concentration (NTG), 25 with primary HTG, and 5 with secondary HTG.
Cross-sectional study using archived samples. Lipoprotein profiles, generated using continuous lipoprotein density profiling, were clustered with hierarchical cluster analysis. Clinical data (age, sex, body condition score, and dietary fat content) was compared between clusters.
Six clusters were identified. Dogs with primary HTG were dispersed among 4 clusters. One cluster showed the highest intensities for triglyceride-rich lipoprotein (TRL) and low-density lipoprotein (LDL) fractions and also included 4 dogs with secondary HTG. Two clusters had moderately high TRL fraction intensities and low-to-intermediate LDL intensities. The fourth cluster had high LDL but variable TRL fraction intensities with equal numbers of NTG and mild HTG dogs. The final 2 clusters comprised only NTG dogs with low TRL intensities and low-to-intermediate LDL intensities. The clusters did not appear to be driven by differences in the clinical data.
The results of this study support a spectrum of lipoprotein phenotypes within Miniature Schnauzers that cannot be predicted by triglyceride concentration alone. Lipoprotein profiling might be useful to determine if subtypes have different origins, clinical consequences, and response to treatment.
迷你雪纳瑞犬普遍存在高甘油三酯血症（HTG），易患危及生命的疾病。针对不同管理策略的反应不一，表明可能存在多种亚型。
假设/目的：通过脂蛋白谱聚类分析，确定并描述迷你雪纳瑞犬的 HTG 亚型。我们假设该品种存在多种原发性 HTG 表型。
20 只血清甘油三酯浓度正常的迷你雪纳瑞犬（NTG）、25 只原发性 HTG 犬和 5 只继发性 HTG 犬。
使用存档样本进行横断面研究。使用连续脂蛋白密度谱分析生成脂蛋白谱，采用层次聚类分析进行聚类。比较聚类间的临床数据（年龄、性别、体况评分和饮食脂肪含量）。
共鉴定出 6 个聚类。原发性 HTG 犬分布于 4 个聚类中。一个聚类显示出最高的甘油三酯丰富脂蛋白（TRL）和低密度脂蛋白（LDL）分数强度，还包括 4 只继发性 HTG 犬。2 个聚类具有中等高的 TRL 分数强度和低至中等 LDL 强度。第四个聚类具有高 LDL 但 TRL 分数强度可变，NTG 和轻度 HTG 犬数量相等。最后 2 个聚类仅由 NTG 犬组成，其 TRL 分数强度低，LDL 分数强度低至中等。聚类似乎不受临床数据差异的影响。
本研究结果支持迷你雪纳瑞犬存在脂蛋白表型谱，不能仅通过甘油三酯浓度来预测。脂蛋白谱分析可能有助于确定亚型是否具有不同的起源、临床后果和对治疗的反应。
