College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Carbohydr Polym. 2013 Apr 2;93(2):380-5. doi: 10.1016/j.carbpol.2013.01.029. Epub 2013 Jan 21.
Collagen peptides grafted sodium alginate (SA-COP) was prepared by reacting alginate with collagen peptide via amide linkage in presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy sulfosuccinimide (NHS). The reaction conditions affected the degree of substitution (DS) were studied including the mass ratio of collagen peptide to sodium alginate, reaction temperature and reaction time. The hydrogen peroxide scavenging activity could be controlled by adjusting the DS, concentration and molecular weight. MTT assay was used to investigate the cell viability of SA-COP. The results indicated that the SA-COP exhibited better cell viability, and with the DS and concentration increasing of SA-COP, cell viability increased. The improved functionalities of the derivative might be explained by peptides characteristics.
胶原肽接枝海藻酸钠（SA-COP）是通过在 1-乙基-(二甲基氨基丙基)碳二亚胺（EDC）和 N-羟基琥珀酰亚胺（NHS）存在的条件下，使海藻酸钠与胶原肽反应，通过酰胺键接枝而成。研究了反应条件对接枝率（DS）的影响，包括胶原肽与海藻酸钠的质量比、反应温度和反应时间。通过调整 DS、浓度和分子量，可以控制过氧化氢清除活性。MTT 法研究了 SA-COP 的细胞活力。结果表明，SA-COP 表现出更好的细胞活力，并且随着 SA-COP 的 DS 和浓度的增加，细胞活力增加。该衍生物功能的改善可能与肽的特性有关。
