School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Chin J Integr Med. 2013 May;19(5):353-9. doi: 10.1007/s11655-013-1442-6. Epub 2013 Mar 15.
To investigate the anti-inflammatory and immunoregulatory effects of Yupingfeng (, YPF) Powder and its components in rats.
A rat chronic bronchitis (CB) model was developed using lipopolysaccharide (LPS) combined with bacillus Calmette Guerin (BCG). YPF, simple recipe Astragalus membranaceus (Fisch.) Bge (AM) and Astragalus membranaceus (Fisch.) Bge plus rhizome of Atractylodes macrocephala Koidz (AM+RA) decoction were administered (intragastric administration, once a day for 21 days) to rats, to prevent and treat CB. Immunoregulatory and anti-inflammatory effects of YPF, AM and AM+RA were tested by serum pharmacology in vitro on splenic lymphocytes of normal rats and alveolar macrophages of CB rats.
Inflammation in the pulmonary tissue and the bronchus of CB rats was significantly reduced in the YPF-treatment groups, AM and AM+RA groups demonstrating the efficacy of YPF. Serum samples collected at different times from rats after administration of YPF, AM and AM+RA demonstrated increased proliferation of splenic lymphocytes with area under the effect curve (AUE) of 552.6%, 336.3% and 452.0%, respectively. Treatment of alveolar macrophages with serum samples in YPF, AM or AM+RA group inhibited interleukin-8 (IL-8) in the cell culture media, and the effect was much better in the YPF group compared with AM or AM+RA group, with a higher maximal effect (Emax, P<0.05) and larger AUE (P <0.01 and P<0.05). Moreover, serum from rats treated with AM or AM+RA had similar efficacy, while the efficiency was lower than that treated with YPF.
YPF demonstrated anti-inflammatory and immunoregulatory effects in a rat model of CB, and timedependent relationships were demonstrated in vitro.
探讨玉屏风（YPF）散及其成分对大鼠的抗炎和免疫调节作用。
采用脂多糖（LPS）联合卡介苗（BCG）建立大鼠慢性支气管炎（CB）模型。YPF、单味黄芪（AM）和黄芪加白术（AM+RA）汤分别灌胃（每天 1 次，共 21 天），预防和治疗 CB。采用血清药理学方法，检测 YPF、AM 和 AM+RA 对正常大鼠脾淋巴细胞和 CB 大鼠肺泡巨噬细胞的免疫调节和抗炎作用。
YPF 治疗组大鼠肺组织和支气管炎症明显减轻，表明 YPF、AM 和 AM+RA 均有疗效。YPF、AM 和 AM+RA 给药后不同时间大鼠血清样本可显著促进脾淋巴细胞增殖，其效应曲线下面积（AUE）分别为 552.6%、336.3%和 452.0%。YPF、AM 或 AM+RA 组血清处理肺泡巨噬细胞可抑制细胞培养上清液中白细胞介素-8（IL-8），YPF 组的作用明显优于 AM 或 AM+RA 组，最大效应（Emax，P<0.05）和 AUE 较大（P<0.01 和 P<0.05）。此外，用 AM 或 AM+RA 处理的血清具有相似的疗效，但效率低于 YPF 处理的血清。
YPF 在 CB 大鼠模型中具有抗炎和免疫调节作用，并在体外显示出时间依赖性关系。
