Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
Mol Imaging Biol. 2013 Aug;15(4):401-10. doi: 10.1007/s11307-013-0622-2.
The aim of this study was to perform the detection of folate receptor (FR)-positive tumors with a bimodal imaging contrast agent, a perfluorocarbon (PFC)/rhodamine nanoemulsion, providing both 19F-based magnetic resonance imaging (MRI) and fluorescence imaging capabilities.
The PFC/rhodamine nanoemulsion was further infused with phospholipid-anchored folate to improve the ability to target FR-expressing tumors. The preferential accumulation of the FR-targeted bimodal nanoemulsion in FR-positive tumor sites was monitored by both 19F-MRI and optical imaging.
The FR-targeted PFC nanoemulsion had no significant effect on cell viability, and the size and fluorescence signal of PFC nanoemulsion were very stable. These nanoprobes were successfully delivered into FR-positive tumor xenograft models and showed significantly enhanced signal intensities of 19F-MRI and fluorescence imaging in the tumor area.
The folate-PFC/rhodamine nanoemulsion has a great potential to serve as a useful optical and 19F-MRI agent for the diagnosis and targeting of FR-positive tumor.
本研究旨在通过一种双模态成像对比剂，即全氟碳（PFC）/罗丹明纳米乳液，实现叶酸受体（FR）阳性肿瘤的检测，该对比剂同时具有基于 19F 的磁共振成像（MRI）和荧光成像功能。
进一步将磷脂锚定叶酸注入 PFC/罗丹明纳米乳液中，以提高靶向 FR 表达肿瘤的能力。通过 19F-MRI 和光学成像监测 FR 靶向双模态纳米乳液在 FR 阳性肿瘤部位的优先积累情况。
FR 靶向 PFC 纳米乳液对细胞活力没有显著影响，并且 PFC 纳米乳液的大小和荧光信号非常稳定。这些纳米探针成功递送至 FR 阳性肿瘤异种移植模型中，并在肿瘤区域显示出 19F-MRI 和荧光成像的信号强度显著增强。
叶酸-PFC/罗丹明纳米乳液有望成为 FR 阳性肿瘤诊断和靶向的有用光学和 19F-MRI 造影剂。
