Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2009 Sep-Oct;1(5):492-501. doi: 10.1002/wnan.35.
In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo(19)F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled cells are introduced into a subject and tracked using (19)F MRI or NMR spectroscopy. In the same imaging session, a high-resolution, conventional ((1)H) image can be used to place the (19)F-labeled cells into anatomical context. Perfluorocarbon-based (19)F cell tracking is a useful technology because of the high specificity for labeled cells, ability to quantify cell accumulations, and biocompatibility. This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy.
本文综述了用于体内(19)F MRI 细胞示踪的全氟碳化合物的化学和纳米乳剂配方。在这种应用中，感兴趣的细胞在培养中使用全氟碳纳米乳剂进行标记。标记的细胞被引入到受试者体内，并使用(19)F MRI 或 NMR 光谱进行跟踪。在同一成像过程中，高分辨率的常规((1)H)图像可用于将(19)F 标记的细胞置于解剖学背景中。基于全氟碳化合物的(19)F 细胞示踪是一种有用的技术，因为它对标记细胞具有高度特异性、能够定量细胞聚集以及具有生物相容性。该技术可广泛应用于炎症、细胞再生医学和免疫治疗的研究。
