Department of Gastroenterology and Metabolism, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
J Med Virol. 2013 May;85(5):789-98. doi: 10.1002/jmv.23540.
Hepatitis B virus (HBV) infection is associated with increased expression of microRNA-122. Serum microRNA-122 and microRNA-22 levels were analyzed in 198 patients with chronic HBV who underwent liver biopsy and were compared with quantitative measurements of HBsAg, HBeAg, HBV DNA, and other clinical and histological findings. Levels of serum microRNA-122 and microRNA-22 were determined by reverse transcription-TaqMan PCR. Serum levels of microRNA-122 and microRNA-22 were correlated (R(2) = 0.576; P < 0.001), and both were elevated in chronic HBV patients. Significant linear correlations were found between microRNA-122 or microRNA-22 and HBsAg levels (R(2) = 0.824, P < 0.001 and R(2) = 0.394, P < 0.001, respectively) and ALT levels (R(2) = 0.498, P < 0.001 and R(2) = 0.528, P < 0.001, respectively). MicroRNA-122 levels were also correlated with HBV DNA titers (R(2) = 0.694, P < 0.001 and R(2) = 0.421, P < 0.001). Levels of these microRNAs were significantly higher in HBeAg-positive patients compared to HBeAg-negative patients (P < 0.001 and P < 0.001). MicroRNA-122 levels were also lower in patients with advanced liver fibrosis (P < 0.001) and lower inflammatory activity (P < 0.025). These results suggest that serum micro-RNA levels are significantly associated with multiple aspects of HBV infection. The biological meaning of the correlation between microRNA-122 and HBsAg and should be investigated further.
乙型肝炎病毒 (HBV) 感染与 microRNA-122 的表达增加有关。对 198 例接受肝活检的慢性 HBV 患者的血清 microRNA-122 和 microRNA-22 水平进行了分析，并与 HBsAg、HBeAg、HBV DNA 等定量测量值以及其他临床和组织学发现进行了比较。通过逆转录- TaqMan PCR 测定血清 microRNA-122 和 microRNA-22 水平。血清 microRNA-122 和 microRNA-22 水平呈正相关（R(2) = 0.576；P<0.001），慢性 HBV 患者均升高。microRNA-122 或 microRNA-22 与 HBsAg 水平（R(2) = 0.824，P<0.001 和 R(2) = 0.394，P<0.001）和 ALT 水平（R(2) = 0.498，P<0.001 和 R(2) = 0.528，P<0.001）均呈显著线性相关。microRNA-122 水平与 HBV DNA 滴度也相关（R(2) = 0.694，P<0.001 和 R(2) = 0.421，P<0.001）。与 HBeAg 阴性患者相比，HBeAg 阳性患者的这些 microRNA 水平显著升高（P<0.001 和 P<0.001）。在肝纤维化程度较高的患者（P<0.001）和炎症活动度较低的患者（P<0.025）中，microRNA-122 水平也较低。这些结果表明，血清 microRNA 水平与 HBV 感染的多个方面显著相关。microRNA-122 与 HBsAg 之间相关性的生物学意义有待进一步研究。
