Department of Earth and Environmental Sciences, Research Centre Polaris, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milano, Italy.
Biomed Res Int. 2013;2013:801214. doi: 10.1155/2013/801214. Epub 2012 Dec 23.
Exposure to engineered nanoparticles (NPs) and to ambient particles (PM) has increased significantly. During the last decades the application of nano-objects to daily-life goods and the emissions produced in highly urbanized cities have considerably augmented. As a consequence, the understanding of the possible effects of NPs and PM on human respiratory system and particularly on the air-blood barrier (ABB) has become of primary interest. The crosstalk between lung epithelial cells and underlying endothelial cells is indeed essential in determining the effects of inhaled particles. Here we report the effects of metal oxides NPs (CuO and TiO2) and of PM on an in vitro model of the ABB constituted by the type II epithelial cell line (NCI-H441) and the endothelial one (HPMEC-ST1.6R). The results demonstrate that apical exposure of alveolar cells induces significant modulation of proinflammatory proteins also in endothelial cells.
暴露于工程纳米颗粒（NPs）和环境颗粒（PM）的情况显著增加。在过去几十年中，纳米物体在日常用品中的应用以及在高度城市化城市中产生的排放大大增加。因此，了解 NPs 和 PM 对人体呼吸系统的可能影响，特别是对气-血屏障（ABB）的影响，已成为首要关注的问题。肺上皮细胞和下面的内皮细胞之间的串扰对于确定吸入颗粒的影响确实很重要。在这里，我们报告了金属氧化物 NPs（CuO 和 TiO2）和 PM 对由 II 型上皮细胞系（NCI-H441）和内皮细胞系（HPMEC-ST1.6R）组成的 ABB 体外模型的影响。结果表明，肺泡细胞的顶端暴露会在内皮细胞中引起促炎蛋白的显著调节。
