Pollin M M, McHanwell S, Slater C R
Division of Neurobiology, School of Neuroscience, Medical School, Newcastle-upon-Tyne, UK.
J Neurocytol. 1990 Feb;19(1):29-38. doi: 10.1007/BF01188437.
This paper describes the location and number of motor neurons in the median nerve pool of wobbler mice and normal littermates as determined by retrograde labelling of the cut median nerve with horseradish peroxidase (HRP) in animals from 3 weeks to 1-year-old. The median nerve motor nucleus is located in spinal segments C5-T1, and in normal animals contains 199 (6) (mean (SEM] motor neurons. Three-week-old wobbler mice have the same number of labelled neurons as control animals, and this number falls to 75% of normal values by 4 weeks of age, and to approximately 60% by 6 weeks of age and older. Numerous swollen, pale and frequently vacuolated perikarya are present in the same 3-6-week-old mice. In the 3-week-old mutants these comprise on average 17% of the total large (greater than 20 microns) neuronal cell bodies counted in segments C5-T1. By 6 weeks this figure has fallen to 10%, and to less than 4% in adult wobblers. We conclude that the most active period in the expression of the wobbler phenotype is from 3 to 6 weeks of age.
本文描述了通过用辣根过氧化物酶（HRP）逆行标记3周龄至1岁动物的正中神经切断端，来确定摇摆小鼠和正常同窝仔鼠正中神经池中运动神经元的位置和数量。正中神经运动核位于脊髓C5-T1节段，正常动物中含有199个（6个）（均值（标准误））运动神经元。3周龄的摇摆小鼠标记神经元数量与对照动物相同，到4周龄时该数量降至正常值的75%，6周龄及以上时降至约60%。在同样3至6周龄的小鼠中存在许多肿胀、苍白且常有空泡的核周体。在3周龄的突变体中，这些核周体平均占C5-T1节段中计数的大型（大于20微米）神经元细胞体总数的17%。到6周龄时，这一比例降至10%，成年摇摆小鼠中则降至不到4%。我们得出结论，摇摆表型表达最活跃的时期是3至6周龄。
