Department of Hospital Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
J Biomed Mater Res A. 2013 Nov;101(11):3076-85. doi: 10.1002/jbm.a.34607. Epub 2013 Mar 25.
In this study, PEGylated nanoparticles quercetin drug delivery vehicles were investigated as carriers for anticancer drugs induced programed cell death (PCD). PEG2000-DPSE-coated quercetin nanoparticles were prepared and tumor cell killing efficacy was studied on glioma C6 cells and assayed for cell survival, apoptosis, or necrosis. The levels of ROS production and mitochondrial membrane potential (ΔΨm) were determined. Western blot assayed p53, p-p53, cytochrome C, and caspase proteins expression were also studied. Results indicate that PEG2000-DPSE-QUE-NPS showed dose-dependent cytotoxicity to C6 glioma cells and enhanced ROS accumulation induced upregulation of p53 protein, which was accompanied with an increase in cytochrome c and caspase-3 protein levels. These results support the hypothesis that quercetin nanoparticles-coated PEG2000-DPSE remarkably enhanced anticancer effect of induced programed cell death on C6 glioma cells. Overall, PEG2000-DPSE-coated quercetin nanoparticles showed promising potential as a drug carrier for cancer therapy.
在这项研究中，聚乙二醇化纳米粒子槲皮素药物传递载体被研究为诱导程序性细胞死亡 (PCD) 的抗癌药物的载体。制备了 PEG2000-DPSE 包裹的槲皮素纳米颗粒，并在神经胶质瘤 C6 细胞上研究了其对肿瘤细胞的杀伤功效，并检测了细胞存活、细胞凋亡或坏死。还测定了 ROS 产生和线粒体膜电位 (ΔΨm) 的水平。Western blot 检测了 p53、p-p53、细胞色素 C 和 caspase 蛋白的表达。结果表明，PEG2000-DPSE-QUE-NPS 对 C6 神经胶质瘤细胞具有剂量依赖性的细胞毒性，并增强了 ROS 积累，诱导 p53 蛋白上调，这伴随着细胞色素 c 和 caspase-3 蛋白水平的增加。这些结果支持了这样一种假设，即聚乙二醇化纳米粒子包裹的槲皮素显著增强了诱导的程序性细胞死亡对 C6 神经胶质瘤细胞的抗癌作用。总的来说，PEG2000-DPSE 包裹的槲皮素纳米颗粒显示出作为癌症治疗药物载体的有前途的潜力。
