Pinheiro D F, Pacheco P D G, Alvarenga P V, Buratini J, Castilho A C S, Lima P F, Sartori D R S, Vicentini-Paulino M L M
Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Braz J Med Biol Res. 2013 Mar;46(3):287-92. doi: 10.1590/1414-431x20122561. Epub 2013 Mar 15.
This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring.
本研究调查了子宫内蛋白质限制对胃肠道的影响，特别是对成年后代肠道双糖酶基因表达和活性的影响。在整个妊娠期，给Wistar大鼠母鼠喂食含6%蛋白质的等热量饮食（限制组，n = 8）或17%蛋白质的等热量饮食（对照组，n = 8）。在3周龄或16周龄时对雄性后代（每组n = 5 - 8）进行评估。孕期母体蛋白质限制使后代从出生（5.7±0.1 vs 6.3±0.1 g；均值±标准误）到断奶（42.4±1.3 vs 49.1±1.6 g）均出现生长受限，不过在16周龄时其体重与对照组相似（421.7±8.9和428.5±8.5 g）。母体蛋白质限制还增加了3周龄后代大鼠近端小肠（0.23±0.02 vs 0.15±0.02）、中间小肠（0.30±0.06 vs 0.14±0.01）和远端小肠（0.43±0.07 vs 0.07±0.02 U·g-1·min-1）的乳糖酶活性，以及近端小肠中乳糖酶mRNA丰度（7.96±1.11 vs 2.38±0.47相对单位）。此外，母体蛋白质限制增加了16周龄大鼠十二指肠中的蔗糖酶活性（1.20±0.02 vs 0.91±0.02 U·g-1·min-1）和蔗糖酶mRNA丰度（4.48±0.51 vs 1.95±0.17相对单位）。总之，本研究首次表明子宫内蛋白质限制会影响后代肠道酶的基因表达。
