Plant Molecular and Cellular Biology, University of Florida, Gainesville, FL 32610, USA.
Proteomics. 2013 Jun;13(12-13):1934-46. doi: 10.1002/pmic.201200402. Epub 2013 Apr 27.
Genetic and microarray analyses have provided useful information in the area of plant and pathogen interactions. Pseudomonas syringae pv. tomato DC3000 (Pst) causes bacterial speck disease in tomato. Previous studies have shown that changes in response to pathogen infection at transcript level are variable at different time points. This study provides information not only on proteomic changes between a resistant and a susceptible genotype, but also information on changes between an early and a late time point. Using the iTRAQ quantitative proteomics approach, we have identified 2369 proteins in tomato leaves, and 477 of them were determined to be responsive to Pst inoculation. Unique and differential proteins after each comparison were further analyzed to provide information about protein changes and the potential functions they play in the pathogen response. This information is applicable not only to tomato proteomics, but also adds to the repertoire of proteins now available for crop proteomic analysis and how they change in response to pathogen infection.
遗传和微阵列分析在植物与病原体相互作用领域提供了有用的信息。丁香假单胞菌 pv.番茄 DC3000（Pst）引起番茄细菌性斑点病。先前的研究表明，转录水平对病原体感染的反应在不同时间点是可变的。本研究不仅提供了抗性和敏感性基因型之间的蛋白质组变化信息，还提供了早期和晚期时间点之间的变化信息。使用 iTRAQ 定量蛋白质组学方法，我们在番茄叶片中鉴定了 2369 种蛋白质，其中 477 种被确定对 Pst 接种有反应。每个比较后的独特和差异蛋白进一步分析，提供了有关蛋白变化及其在病原体反应中潜在功能的信息。这些信息不仅适用于番茄蛋白质组学，而且还增加了现在可用于作物蛋白质组分析的蛋白质谱以及它们如何响应病原体感染而变化的信息。
