Wetland Research and Training Centre, Chilika Development Authority, Balugaon, Odisha, 752030, India.
School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
J Microbiol. 2018 Jul;56(7):458-466. doi: 10.1007/s12275-018-7549-x. Epub 2018 Jun 14.
The taxonomic position of a Gram-stain-negative, rod-shaped bacterial strain, designated PI11, isolated from the rhizospheric sediment of Phragmites karka was characterized using a polyphasic approach. Strain PI11 could grow optimally at 1.0% NaCl concentration with pH 7.0 at 30°C and was positive for oxidase and catalase but negative for hydrolysis of starch, casein, and esculin ferric citrate. Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain PI11 belonged to the genus Pseudomonas sharing the highest sequence similarities with Pseudomonas indoloxydans JCM 14246 (99.72%), followed by, Pseudomonas oleovorans subsp. oleovorans DSM 1045 (99.29%), Pseudomonas toyotomiensis JCM 15604 (99.15%), Pseudomonas chengduensis DSM 26382 (99.08%), Pseudomonas oleovorans subsp. lubricantis DSM 21016 (99.08%), and Pseudomonas alcaliphila JCM 10630 (99.01%). Experimental DNA-DNA relatedness between strain PI11 and P. indoloxydans JCM 14246 was 49.4%. The draft genome of strain PI11 consisted of 4,884,839 bp. Average nucleotide identity between the genome of strain PI11 and other closely related type strains ranged between 77.25-90.74%. The polar lipid pattern comprised of phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine. The major (> 10%) cellular fatty acids were Cω6c/ω7c, Cω6c/ω7c, and C. The DNA G + C content of strain PI11 was 62.4 mol%. Based on the results of polyphasic analysis, strain PI11 was delineated from other closely related type strains. It is proposed that strain PI11 represents represents a novel species of the genus Pseudomonas, for which the name Pseudomonas sediminis sp. nov. is proposed. The type strain is PI11 (= KCTC 42576 = DSMZ 100245).
一株革兰氏阴性、杆状细菌菌株的分类地位，被命名为 PI11，从 Phragmites karka 的根际沉积物中分离出来，通过多相研究进行了描述。菌株 PI11 可以在 1.0%NaCl 浓度、pH7.0 和 30°C 的最佳条件下生长，并且氧化酶和过氧化氢酶呈阳性，但淀粉、酪蛋白和柠檬酸铁 Esculin 的水解呈阴性。16S rRNA 基因序列的系统发育分析表明，该菌株 PI11 属于假单胞菌属，与 Pseudomonas indoloxydans JCM 14246 的序列相似度最高（99.72%），其次是 Pseudomonas oleovorans subsp. oleovorans DSM 1045（99.29%）、Pseudomonas toyotomiensis JCM 15604（99.15%）、Pseudomonas chengduensis DSM 26382（99.08%）、Pseudomonas oleovorans subsp. lubricantis DSM 21016（99.08%）和 Pseudomonas alcaliphila JCM 10630（99.01%）。菌株 PI11 与 Pseudomonas indoloxydans JCM 14246 之间的实验性 DNA-DNA 相关性为 49.4%。菌株 PI11 的基因组草案由 4884839 个碱基对组成。菌株 PI11 与其他密切相关的模式菌株之间的平均核苷酸同一性在 77.25-90.74%之间。极性脂谱由磷脂酰甘油、双磷脂酰甘油和磷脂酰胆碱组成。主要（>10%）细胞脂肪酸为 Cω6c/ω7c、Cω6c/ω7c 和 C。菌株 PI11 的 DNA G+C 含量为 62.4mol%。基于多相分析的结果，菌株 PI11 与其他密切相关的模式菌株区分开来。建议菌株 PI11 代表假单胞菌属的一个新种，命名为 Pseudomonas sediminis sp. nov.。模式菌株为 PI11（=KCTC 42576=DSMZ 100245）。
