Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
Mycotoxin Res. 2013 Aug;29(3):169-75. doi: 10.1007/s12550-013-0166-y. Epub 2013 Mar 28.
Fusarium toxin-contaminated ground maize was hydrothermally treated in the presence of different combinations of chemicals in order to simultaneously reduce zearalenone (ZEA) and deoxynivalenol (DON) concentrations. Treatments were carried out in a laboratory conditioner at 80 °C and 17 % moisture. Six different treatments were performed, consisting of 3 doses of methylamine (MMA; 2.5, 5 and 10 g/kg maize) at a constant dose of 5 g sodium metabisulfite (SBS)/kg, either with or without the addition of 20 g calcium hydroxide (Ca(OH)2)/kg. The used maize was contaminated with approximately 45.99 mg DON/kg and 3.46 mg ZEA/kg. Without the addition of Ca(OH)2, DON reductions reached approximately 82% after 1-min treatment and the toxin disappeared nearly completely after 10 min when 2.5 or 5 g MMA were applied. ZEA concentrations were only marginally affected. In the presence of Ca(OH)2, reductions in DON concentrations were lower, but were enhanced by increasing doses of MMA. ZEA concentrations were reduced by 72, 85 and 95% within the first 5 min of the treatment at MMA dosages of 2.5, 5 and 10 g/kg maize, respectively. The application of SBS in combination with a strong alkaline during hydrothermal treatment seems to be a promising approach to simultaneously decontaminate even high amounts of DON and ZEA in ground maize and may contribute to reduce the toxin load of diets.
镰刀菌毒素污染的地面玉米在不同化学物质组合的存在下进行水热处理，以同时降低玉米赤霉烯酮（ZEA）和脱氧雪腐镰刀菌烯醇（DON）的浓度。处理在 80°C 和 17%水分的实验室调温器中进行。进行了六种不同的处理，由 3 种甲胺（MMA；2.5、5 和 10 g/kg 玉米）剂量组成，在恒定剂量的 5 g 亚硫酸氢钠（SBS）/kg 下，要么添加，要么不添加 20 g 氢氧化钙（Ca(OH)2）/kg。所用玉米污染了约 45.99 mg DON/kg 和 3.46 mg ZEA/kg。在不添加 Ca(OH)2 的情况下，经过 1 分钟处理后，DON 的减少量约为 82%，当添加 2.5 或 5 g MMA 时，毒素几乎在 10 分钟后完全消失。ZEA 浓度仅受到轻微影响。在 Ca(OH)2 的存在下，DON 浓度的降低幅度较小，但随着 MMA 剂量的增加而增强。在 MMA 剂量分别为 2.5、5 和 10 g/kg 玉米的情况下，在处理的前 5 分钟内，ZEA 浓度分别降低了 72%、85%和 95%。在水热处理中同时使用 SBS 和强碱性物质似乎是一种很有前途的方法，可以同时去除地面玉米中高含量的 DON 和 ZEA，并且可能有助于降低饲料中的毒素负荷。
