Kum-Tatt L, Tan I K, Seet A M
Clin Chim Acta. 1975 Jan 20;58(2):101-8. doi: 10.1016/s0009-8981(75)80002-7.
A simple and rapid colorimetric method for the assay of erythrocyte and plasma glutathione reductase (GR) activity is described. The method is based on the colorimetric measurement of reduced glutathione (GSH) (1) produced when the enzyme is incubated with oxidised glutathione (GSSG) in the presence of either reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH). Results of investigations on the effects of substrate and coenzyme concentrations, pH, EDTA, sodium/potassium chloride, and time, on enzyme activity are presented. Erythrocyte and plasma NADH-GR and NADPH-GR activities in 100 healthy blood donors, and 85 cord blood samples and plasma NADH-GR and NADPH-GR levels in patients with various disease conditions are given.
本文描述了一种简单快速的比色法，用于测定红细胞和血浆谷胱甘肽还原酶（GR）活性。该方法基于在还原型烟酰胺腺嘌呤二核苷酸（NADH）或还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在下，当酶与氧化型谷胱甘肽（GSSG）孵育时产生的还原型谷胱甘肽（GSH）（1）的比色测量。文中给出了关于底物和辅酶浓度、pH值、乙二胺四乙酸（EDTA）、氯化钠/氯化钾以及时间对酶活性影响的研究结果。还给出了100名健康献血者的红细胞和血浆NADH - GR以及NADPH - GR活性，85份脐带血样本，以及各种疾病患者的血浆NADH - GR和NADPH - GR水平。
