Faculty of Science, University of South Bohemia, and Institute of Parasitology, Biology Centre, ASCR, v.v.i., Branisovka 31, 37005 Ceske Budejovice, Czech Republic.
Mol Phylogenet Evol. 2013 Jul;68(1):42-54. doi: 10.1016/j.ympev.2013.03.016. Epub 2013 Mar 29.
Reliable phylogenetic reconstruction, as a framework for evolutionary inference, may be difficult to achieve in some groups of organisms. Particularly for lineages that experienced rapid diversification, lack of sufficient information may lead to inconsistent and unstable results and a low degree of resolution. Coincidentally, such rapidly diversifying taxa are often among the biologically most interesting groups. Aphids provide such an example. Due to rapid adaptive diversification, they feature variability in many interesting biological traits, but consequently they are also a challenging group in which to resolve phylogeny. Particularly within the family Aphididae, many interesting evolutionary questions remain unanswered due to phylogenetic uncertainties. In this study, we show that molecular data derived from the symbiotic bacteria of the genus Buchnera can provide a more powerful tool than the aphid-derived sequences. We analyze 255 Buchnera gene sequences from 70 host aphid species and compare the resulting trees to the phylogenies previously retrieved from aphid sequences, only. We find that the host and symbiont data do not conflict for any major phylogenetic conclusions. Also, we demonstrate that the symbiont-derived phylogenies support some previously questionable relationships and provide new insights into aphid phylogeny and evolution.
可靠的系统发育重建作为进化推断的框架，在某些生物群体中可能难以实现。特别是对于经历快速多样化的谱系，缺乏足够的信息可能导致不一致和不稳定的结果以及低分辨率。巧合的是，这种快速多样化的分类群通常是生物学上最有趣的群体之一。蚜虫就是一个例子。由于快速的适应性多样化，它们具有许多有趣的生物特征的可变性，但因此它们也是一个难以解决系统发育的群体。特别是在蚜科中，由于系统发育的不确定性，许多有趣的进化问题仍然没有答案。在这项研究中，我们表明，来源于共生菌 Buchnera 的分子数据比蚜虫衍生的序列提供了更强大的工具。我们分析了来自 70 种宿主蚜虫物种的 255 个 Buchnera 基因序列，并将生成的树与仅从蚜虫序列中检索到的系统发育进行了比较。我们发现，对于任何主要的系统发育结论，宿主和共生体数据都没有冲突。此外，我们证明了共生体衍生的系统发育支持一些以前有问题的关系，并为蚜虫的系统发育和进化提供了新的见解。
