Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA.
Lab Chip. 2013 Jul 21;13(14):2679-81. doi: 10.1039/c3lc50207d.
We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.
我们使用通过长度可调肽连接的基因编码荧光蛋白 FRET 对实现了光流体蛋白激光。与使用传统 FRET 检测时仅 17%的供体发射减少相比，当供体和受体接近时，观察到供体激光发射减少了 25 倍。我们的工作为研究蛋白质-蛋白质相互作用和蛋白质-药物相互作用打开了一扇广阔的应用之门。
