Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory of Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, China.
J Anim Sci. 2019 Mar 1;97(3):1133-1142. doi: 10.1093/jas/skz031.
Displacement of abomasum (DA) is one of the most common and important disorders in dairy cattle. The objective of the present study was to detect the quantitative trait loci (QTL) for DA in Chinese Holstein using single-step genomic BLUP methodology. A total of 60,556 producer-recorded DA event records from 32,190 cows, together with 2,336 genotyped animals with 40,054 SNP markers, were used for the analysis. Genomic data were incorporated into a threshold model for variance component estimation, and the estimated heritability of DA was 0.108 (SE = 0.086). Results of genome-wide association studies were reported as the proportion of genetic variance explained 20-SNP windows. Eight QTLs covering 129 genes on Bos taurus autosomes 2, 4, 7, 10, 14, 17, 20 showed associations with DA. Ten genes, namely BMP4, SOCS4, GCH1, DDHD1, ATG14, ACBP/DBI, SMO, AHCYL2, CYP7A1, and CACNA1A, involved in insulin metabolism and lipid metabolism pathways may be considered as candidate genes of DA in dairy. The identified QTLs, biological pathways, and associated genes underlying DA identified from the present study will contribute to the understanding of the genetic architecture of this complex disease.
真胃移位（DA）是奶牛中最常见和最重要的疾病之一。本研究旨在使用一步法基因组 BLUP 方法检测中国荷斯坦奶牛 DA 的数量性状位点（QTL）。使用了来自 32190 头奶牛的 60556 个生产者记录的 DA 事件记录，以及 2336 个具有 40054 个 SNP 标记的基因分型动物，用于分析。基因组数据被纳入方差分量估计的阈值模型，DA 的估计遗传力为 0.108（SE = 0.086）。全基因组关联研究的结果以解释 20-SNP 窗口遗传方差的比例报告。在牛 Taurus 染色体 2、4、7、10、14、17、20 上，涵盖 129 个基因的 8 个 QTL 与 DA 相关。10 个基因，即 BMP4、SOCS4、GCH1、DDHD1、ATG14、ACBP/DBI、SMO、AHCYL2、CYP7A1 和 CACNA1A，涉及胰岛素代谢和脂质代谢途径，可被视为奶牛 DA 的候选基因。本研究从 DA 中鉴定出的 QTL、生物途径和相关基因将有助于理解这种复杂疾病的遗传结构。
