Gao Yan, Li Fujuan, Zhang Yonghong, Dai Lisheng, Jiang Hao, Liu Huiyu, Zhang Shumin, Chen Chengzhen, Zhang Jiabao
College of Animal Sciences, Jilin University, Changchun 130062, China.
Cell Physiol Biochem. 2013;31(2-3):452-61. doi: 10.1159/000343381. Epub 2013 Mar 15.
Our study aims to characterize the functions of the ADIPOQ gene in the process of fat deposition of pigs, thereby providing a basis for the use of this gene as a molecular marker for pork quality.
We used healthy Junmu1 piglets less than 7 days of age to establish an in vitro culture system for porcine preadipocytes. Chemically synthesized short hairpin RNAs (shRNA) were transfected into porcine preadipocytes to silence the expression of the ADIPOQ gene. We monitored preadipocyte differentiation and determined the levels of the adipocyte differentiation transcription factors lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid binding protein (AP2) mRNAs to investigate the effects of ADIPOQ on the differentiation of porcine preadipocytes.
After transfection, the mRNA and protein levels of the ADIPOQ gene were significantly decreased (P < 0.01), the number of lipid droplets in the adipocytes was significantly reduced, the OD values reflecting the fat content were significantly decreased (P < 0.01), and the levels of LPL, PPARγ and AP2 were significantly reduced (P < 0.01).
These results suggest that interference with ADIPOQ gene expression can inhibit the differentiation of porcine preadipocytes.
本研究旨在表征ADIPOQ基因在猪脂肪沉积过程中的功能，从而为将该基因用作猪肉品质分子标记提供依据。
我们使用7日龄以下的健康军牧1号仔猪建立猪前体脂肪细胞体外培养体系。将化学合成的短发夹RNA（shRNA）转染到猪前体脂肪细胞中，以沉默ADIPOQ基因的表达。我们监测前体脂肪细胞分化，并测定脂肪细胞分化转录因子脂蛋白脂肪酶（LPL）、过氧化物酶体增殖物激活受体γ（PPARγ）和脂肪细胞脂肪酸结合蛋白（AP2）的mRNA水平，以研究ADIPOQ对猪前体脂肪细胞分化的影响。
转染后，ADIPOQ基因的mRNA和蛋白水平显著降低（P<0.01），脂肪细胞中脂滴数量显著减少，反映脂肪含量的OD值显著降低（P<0.01），LPL、PPARγ和AP2水平显著降低（P<0.01）。
这些结果表明，干扰ADIPOQ基因表达可抑制猪前体脂肪细胞的分化。
