State Key Laboratory of Rare Earth Resources Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, China.
Biomaterials. 2013 Jul;34(20):4810-7. doi: 10.1016/j.biomaterials.2013.03.039. Epub 2013 Mar 30.
We have demonstrated a robust sensing strategy by employing single-stranded probe DNA and the hemin-graphene hybrid (GH) to detect a broad range of targets including metal ions, DNA and small molecules. This nearly "universal" biosensor approach is based on the DNA-mediated assembly of the hemin-graphene composite upon addition of the targets. Afterwards, GH aggregate resulting from DNA hybridization will occur. The DNA-GH hybrids will settle on the bottom of the vial after centrifugation, leaving behind a transparent supernatant. After incubation with TMB and H2O2, the colorimetric signal of the centrifugal supernatant will be significantly lower compared to that in the absence of targets. Therefore, mediation of the assembly of DNA on GH by targets can yield a facile means with tunable optical properties in response to concentration changes of the targets. This colorimetric "readout" offers great advantages such as the simple operation process, low-cost portable instrument and easy-to-use applications. Therefore, we believe that this method promises a great potential of becoming a routine tool for quantitative detection of a wide spectrum of analytes for specific applications in biodiagnostics, nanoelectronics, and bionanotechnology.
我们通过使用单链探针 DNA 和血红素-石墨烯杂化 (GH) 展示了一种强大的传感策略，以检测包括金属离子、DNA 和小分子在内的广泛目标。这种几乎“通用”的生物传感器方法基于在添加目标后通过 DNA 介导的血红素-石墨烯复合物的组装。随后，将发生由于 DNA 杂交而导致的 GH 聚集体。GH 混合物在离心后将沉淀在小瓶底部，留下透明的上清液。与 TMB 和 H2O2 孵育后，与不存在目标相比，离心上清液的比色信号将显著降低。因此，目标介导的 DNA 在 GH 上的组装可以提供一种简单的方法，具有可调谐的光学性质，以响应目标浓度的变化。这种比色“读出”具有操作过程简单、低成本便携式仪器和易于使用等优点。因此，我们相信这种方法有望成为一种常规工具，用于定量检测生物诊断、纳米电子学和生物纳米技术等特定应用中广泛的分析物。
