Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
FEMS Microbiol Ecol. 2013 Aug;85(2):348-57. doi: 10.1111/1574-6941.12122. Epub 2013 Apr 18.
The formation of biofilms and biofouling is a common feature in aquatic environments. The aim of this study was to identify the primary colonizers of biofilm formed in Eastern Mediterranean Coastal water at different seasons and follow early dynamics of biofilm community development. Pre-treated coastal seawater and biofilm samples were collected from six different sampling events of 2 weeks' duration each during 1 year. The microbial community composition and specific abundance were estimated by 16S rRNA gene clone libraries and fluorescence in situ hybridization-confocal laser scanning microscopy (FISH-CLSM), respectively. The biofilm formed over the course of the year was fairly consistent in terms of community composition and overall abundance with the exception of spring season. Alphaproteobacteria (30-70% of total bacteria), in particular Rhodobacteraceae, were the dominant bacteria in the biofilm, regardless of season, followed by Bacteroidetes (5-35%) and Gammaproteobacteria (6-35%). There was a decrease in relative abundance of Alphaproteobacteria and an increase in the abundance of Bacteroidetes between the initial and 2-week-old biofilm. This observation may aid man-made facilities that have to deal with biofilm formation and help the development of appropriate strategies to control those biofilms.
生物膜和生物污垢的形成是水生环境中的一个共同特征。本研究的目的是确定在地中海东部沿海不同季节形成的生物膜的主要定植者，并跟踪生物膜群落发展的早期动态。在一年中，每个季节进行为期两周的六次采样，采集预处理过的沿海海水和生物膜样本。通过 16S rRNA 基因克隆文库和荧光原位杂交-共聚焦激光扫描显微镜（FISH-CLSM）分别估计微生物群落组成和特定丰度。除春季外，全年形成的生物膜在群落组成和总丰度方面相当一致。无论季节如何，α变形菌（占总细菌的 30-70%），特别是红杆菌科，都是生物膜中的主要细菌，其次是拟杆菌门（5-35%）和γ变形菌门（6-35%）。在初始生物膜和 2 周龄生物膜之间，α变形菌的相对丰度下降，而拟杆菌门的丰度增加。这一观察结果可能有助于处理生物膜形成的人造设施，并有助于制定控制这些生物膜的适当策略。
