Princeton University, Department of Electrical Engineering, OldenStreet, Princeton, New Jersey 08544, USA.
J Biomed Opt. 2013 Apr;18(4):040503. doi: 10.1117/1.JBO.18.4.040503.
We have developed a microfluidic device that enables the computation of three-dimensional (3-D) images of flowing samples. Using a microfluidic channel that is tilted along the optical axis, we record several progressively defocused images of the flowing sample as it passes across the focal plane. The resulting focal stack is then deconvolved to generate 3-D images. Experimental results on flowing yeast cells reveal both volume and surface profile information. The microfluidic channel eliminates the need for a precise translation stage to control defocusing and enables high sample throughput in an insulated, nontoxic, liquid environment. The experimental device can be implemented in all existing microscopes as a modified slide stage and is ideally suited for 3-D profiling in flow cytometers.
我们开发了一种微流控装置，能够对流动样本进行三维（3-D）图像计算。我们使用沿光轴倾斜的微流道，在流动样本穿过焦平面时记录几个逐渐失焦的样本图像。然后对所得的焦堆叠进行反卷积以生成 3-D 图像。对流动酵母细胞的实验结果揭示了体积和表面轮廓信息。微流道消除了对精确平移台的需求，以控制离焦，并且能够在绝缘、无毒的液体环境中实现高通量样本。实验装置可以作为改装的载物台在所有现有的显微镜中实现，非常适合在流式细胞仪中进行 3-D 轮廓分析。
