Centre d'Immunologie de Marseille-Luminy, UNIV UM2, Aix-Marseille Université, Parc scientifique et technologique de Luminy, Marseille, France.
Eur J Immunol. 2013 Jul;43(7):1706-15. doi: 10.1002/eji.201243106.
DCs express receptors sensing microbial, danger or cytokine signals, which when triggered in combination drive DC maturation and functional polarization. Maturation was proposed to result from a discrete number of modifications in conventional DCs (cDCs), in contrast to a cell-fate conversion in plasmacytoid DCs (pDCs). cDC maturation is generally assessed by measuring cytokine production and membrane expression of MHC class II and co-stimulation molecules. pDC maturation complexity was demonstrated by functional genomics. Here, pDCs and cDCs were shown to undergo profound and convergent changes in their gene expression programs in vivo during viral infection. This observation was generalized to other stimulation conditions and DC subsets, by public microarray data analyses, PCR confirmation of selected gene expression profiles, and gene regulatory sequence bioinformatics analyses. Thus, maturation is a complex process similarly reshaping all DC subsets, including through the induction of a core set of NF-κB- or IFN-stimulated genes irrespective of stimuli.
树突状细胞 (DCs) 表达能够感知微生物、危险或细胞因子信号的受体，这些信号的组合触发会导致 DC 成熟和功能极化。成熟被认为是传统树突状细胞 (cDCs) 中一系列离散修饰的结果，而不是浆细胞样树突状细胞 (pDCs) 中的细胞命运转换。cDC 成熟通常通过测量细胞因子的产生和 MHC Ⅱ类和共刺激分子的膜表达来评估。通过功能基因组学证明了 pDC 成熟的复杂性。在这里，在病毒感染期间，体内研究表明 pDCs 和 cDCs 的基因表达程序发生了深刻而趋同的变化。通过公共微阵列数据分析、对选定基因表达谱的 PCR 确认以及基因调控序列生物信息学分析，将这一观察结果推广到其他刺激条件和 DC 亚群。因此，成熟是一个复杂的过程，同样重塑了所有的 DC 亚群，包括通过诱导一组核心的 NF-κB 或 IFN 刺激基因，而与刺激无关。
