Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA.
Nat Immunol. 2012 Sep;13(9):888-99. doi: 10.1038/ni.2370. Epub 2012 Jul 15.
Although much progress has been made in the understanding of the ontogeny and function of dendritic cells (DCs), the transcriptional regulation of the lineage commitment and functional specialization of DCs in vivo remains poorly understood. We made a comprehensive comparative analysis of CD8(+), CD103(+), CD11b(+) and plasmacytoid DC subsets, as well as macrophage DC precursors and common DC precursors, across the entire immune system. Here we characterized candidate transcriptional activators involved in the commitment of myeloid progenitor cells to the DC lineage and predicted regulators of DC functional diversity in tissues. We identified a molecular signature that distinguished tissue DCs from macrophages. We also identified a transcriptional program expressed specifically during the steady-state migration of tissue DCs to the draining lymph nodes that may control tolerance to self tissue antigens.
尽管人们对树突状细胞 (DC) 的个体发生和功能有了很多了解，但体内 DC 谱系定向和功能特化的转录调控仍知之甚少。我们对整个免疫系统中的 CD8(+)、CD103(+)、CD11b(+) 和浆细胞样 DC 亚群，以及巨噬细胞 DC 前体和普通 DC 前体进行了全面的比较分析。在这里，我们鉴定了参与髓系祖细胞向 DC 谱系定向的候选转录激活因子，并预测了组织中 DC 功能多样性的调节因子。我们确定了一个可以区分组织 DC 和巨噬细胞的分子特征。我们还鉴定了一个在组织 DC 向引流淋巴结的稳态迁移过程中特异性表达的转录程序，该程序可能控制对自身组织抗原的耐受。
