Wei Muxin, Wu Yanmin, Chen Dezheng, Gu Yuchun
Department of Traditional Chinese Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, Jingsu Province, China.
J Biomed Res. 2010 May;24(3):250-5. doi: 10.1016/S1674-8301(10)60035-8.
To explore the nature of deficiency in spleen-yin syndrome, which could provide scientific theoretical support and practical guidance for clinical Traditional Chinese Medicine (TCM) syndrome differentiation based on biology, and had a strong clinical significance.
Serum Cu and Zn were detected by atomic absorption spectrophotometer, serum vitamin E by high performance liquid chromatography, serum vitamin C by 2,4-Dinitrophenylhydrazine Colorimetry, total superoxide dismutase (SOD) and Cu and Zn-SOD by the xanthine oxidase method, and malondialdehyde (MDA) by the 2-thiobarbituric acid method (TBA). Total antioxidant capacity was detected by a colorimetry kit. Amylase Activity was detected by an automatic biochemical analyzer. Lysozyme was detected by lysozyme detection plate, the diameter of bacteriolysis circle was measured and the corresponding content of lysozyme was obtained from a table of standard curve values.
No significant difference in total SOD and Cu, Zn-SOD was found between deficiency in spleen-yin group and normal group. However, such factors in deficiency in kidney-yin group were significantly lower than the other groups (P < 0.05). The MDA content in both deficiency in spleen-yin group and deficiency in kidney-yin group were significantly higher than that of normal group (P < 0.05), while the total antioxidant capacity was significantly lower than normal group (P < 0.05). The vitamin E content in deficiency in kidney-yin group was significantly lower than that in the other two groups (P < 0.05). No significant difference in the contents of vitamin C, Cu and Zn were observed in these groups. The Zn/Cu level in deficiency in kidney-yin group and the vitamin E level in deficiency in spleen-yin group decreased, but with no significant difference. Amylase activity in unit time in cases with deficiency in spleen-yin was lower than and had significant differences with that in normal cases, and higher than that in cases with deficiency in kidney-yin. The sectional velocity of saliva and the ratio of lysozyme in normal case group were significantly higher than other two groups, while deficiency in the spleen-yin group was significantly higher than the deficiency in kidney-yin group.
All the results indicated that the objective pathological mechanism between the deficiency in spleen-yin and deficiency in kidney-yin was different.
探讨脾阴虚证的本质，为中医临床辨证提供生物学依据，具有较强的临床指导意义。
采用原子吸收分光光度计检测血清铜、锌，高效液相色谱法检测血清维生素E，2,4-二硝基苯肼比色法检测血清维生素C，黄嘌呤氧化酶法检测总超氧化物歧化酶（SOD）、铜锌超氧化物歧化酶（Cu,Zn-SOD），硫代巴比妥酸法（TBA）检测丙二醛（MDA）。采用比色法试剂盒检测总抗氧化能力。采用自动生化分析仪检测淀粉酶活性。采用溶菌酶检测平板检测溶菌酶，测量抑菌圈直径，根据标准曲线值表得出相应的溶菌酶含量。
脾阴虚组与正常组总SOD、Cu,Zn-SOD比较，差异无统计学意义；肾阴虚组上述指标明显低于其他组（P<0.05）。脾阴虚组和肾阴虚组MDA含量均明显高于正常组（P<0.05），总抗氧化能力明显低于正常组（P<0.05）。肾阴虚组维生素E含量明显低于其他两组（P<0.05）。三组维生素C、铜、锌含量比较，差异无统计学意义。肾阴虚组锌/铜比值、脾阴虚组维生素E水平降低，但差异无统计学意义。脾阴虚患者单位时间淀粉酶活性低于正常组，差异有统计学意义，高于肾阴虚组。正常组唾液分段速度、溶菌酶比值明显高于其他两组，脾阴虚组明显高于肾阴虚组。
结果提示，脾阴虚证与肾阴虚证的客观病理机制不同。
