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用高效薄层色谱法（HPTLC）分析蓝星花的次生代谢产物特征

Secondary metabolite credentials of Evolvulus alsinoides by high performance thin layer chromatography (HPTLC).

作者信息

Gomathi Duraisamy, Kalaiselvi Manokaran, Ravikumar Ganesan, Sophia Dominic, Gopalakrishnan Velliyur Kanniappan, Uma Chandrasekar

机构信息

Department of Biochemistry, Karpagam University, Coimbatore 641021, India.

出版信息

J Biomed Res. 2012 Jul;26(4):295-302. doi: 10.7555/JBR.26.20110128. Epub 2012 Jul 7.

DOI:10.7555/JBR.26.20110128
PMID:23554763
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3596747/
Abstract

Plants and plant-based products are the bases of many modern pharmaceuticals that are current in use today for various diseases. The aim of the study was to investigate the biochemical constituents and high performance thin layer chromatography (HPTLC) finger printing of the ethanolic extract of Evolvulus alsinoides. Phytochemical screening was done by standard procedures and HPTLC method was also established to analyze alkaloids, flavonoids and phenolic compounds from the ethanolic extract of Evolvulus alsinoides. Preliminary phytochemical screening showed that ethanol extracted more secondary metabolites than other solvents. HPTLC fingerprinting analysis showed the presence of various alkaloids, flavonoids and phenols (quercetin) in the ethanolic extract. It can be concluded that Evolvulus alsinoides may serve as a source of potent antioxidants that may be used in the prevention of various diseases such as cancer, diabetes and cardiovascular diseases due to the presence of phenolic compounds. HPTLC finger print of Evolvulus alsinoides may be useful in the differentiation of the species from adulterants and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry and plant systematic studies.

摘要

植物及植物基产品是当今许多用于治疗各种疾病的现代药物的基础。本研究的目的是调查耳草的乙醇提取物的生化成分及高效薄层色谱（HPTLC）指纹图谱。采用标准程序进行植物化学筛选，并建立HPTLC方法以分析耳草乙醇提取物中的生物碱、黄酮类化合物和酚类化合物。初步植物化学筛选表明，乙醇提取的次生代谢产物比其他溶剂更多。HPTLC指纹图谱分析表明，乙醇提取物中存在各种生物碱、黄酮类化合物和酚类（槲皮素）。可以得出结论，由于酚类化合物的存在，耳草可能作为一种有效的抗氧化剂来源，可用于预防各种疾病，如癌症、糖尿病和心血管疾病。耳草的HPTLC指纹图谱可能有助于区分该物种与掺假品，并在制药行业和植物系统研究中作为这种具有重要药用价值的植物的生化标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/cd09967644f3/jbr-26-04-295-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/949e56d9fef9/jbr-26-04-295-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/d41ba50305c7/jbr-26-04-295-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/ed8fffdefdb9/jbr-26-04-295-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/e83ce1ddd1d2/jbr-26-04-295-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/549f44fb41f5/jbr-26-04-295-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/cd09967644f3/jbr-26-04-295-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/949e56d9fef9/jbr-26-04-295-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/d41ba50305c7/jbr-26-04-295-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/ed8fffdefdb9/jbr-26-04-295-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/e83ce1ddd1d2/jbr-26-04-295-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/549f44fb41f5/jbr-26-04-295-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/3596747/cd09967644f3/jbr-26-04-295-g006.jpg

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