Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou 510006, China ; School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou 510006, China.
Evid Based Complement Alternat Med. 2013;2013:340267. doi: 10.1155/2013/340267. Epub 2013 Feb 28.
The protein-enriched extracts of housefly larvae were segregated by gel-filtration chromatography (GFC) and then anti-inflammatory activity screening in RAW264.7 (induced by LPS) was carried out. After acquire the anti-inflammatory effective parts, its anti-atherosclerotic properties in vivo were then evaluated. Results showed that the anti-inflammatory effective parts of housefly larvae were low-molecular-weight parts. After treated with the effective parts oral gavaged for 4 weeks, the atherosclerotic lesions of the mouse were significantly decreased. The inflammatory and lipid parameters were also reduced (except HDL which was increased). Western blot analysis demonstrated that the effective parts exerted potent inhibitory effect on expression of p65 in nucleus and cytoplasm. The results of immunofluorescence microscopy analysis also showed that the expressions of p65 both in cytoplasm and nucleus were significantly reduced. The hypothesis that the anti-inflammatory effective parts of housefly larvae possessed anti-atherosclerosis activity in mouse and the possible mechanism could be associated with the inhibition of expression and nuclear transfer of NF- κ B p65 could be derived.
家蝇幼虫的富含蛋白质的提取物通过凝胶过滤色谱（GFC）进行分离，然后在 RAW264.7（由 LPS 诱导）中进行抗炎活性筛选。获得抗炎有效部位后，评估其在体内的抗动脉粥样硬化特性。结果表明，家蝇幼虫的抗炎有效部位为低分子量部分。用有效部位口服灌胃 4 周后，小鼠的动脉粥样硬化病变明显减少。炎症和脂质参数也降低（除了 HDL 升高）。Western blot 分析表明，有效部位对核和细胞质中 p65 的表达具有很强的抑制作用。免疫荧光显微镜分析的结果也表明，细胞质和细胞核中 p65 的表达均显著降低。可以得出这样的假设：家蝇幼虫的抗炎有效部位在小鼠中具有抗动脉粥样硬化活性，其可能的机制可能与抑制 NF- κ B p65 的表达和核转移有关。
