Berk L S, Nieman D C, Youngberg W S, Arabatzis K, Simpson-Westerberg M, Lee J W, Tan S A, Eby W C
Department of Pathology and Laboratory Medicine, Loma Linda University, CA 92350.
Med Sci Sports Exerc. 1990 Apr;22(2):207-12.
Ten experienced marathoners were exercised 3 h in the laboratory. Blood samples were collected at 0 h baseline, 1 h exercise, and 5 min, 1.5 h, 6 h, and 21 h recovery and were analyzed for total number of lymphocytes expressing membrane antigens found on natural killer (NK) cells. NK activity was also measured. Four of the seven subpopulations of lymphocytes studied, Leu-11+19+, Leu-11+19-, Leu-11+7-, and Leu-19+11-, showed significant within-subject effects over time, using repeated measures ANOVA. Simple contrasts with baseline values showed that, at 1.5 h and 21 h recovery, total number of lymphocytes bearing three different combinations of NK markers, Leu-11+19+, Leu-11+19-, and Leu-11+7-, were significantly decreased when compared with baseline values. At 1.5 h recovery, NK activity was significantly decreased below baseline levels for four of the six effector NK cell/target K562 myelogenous leukemia cell (E:T) ratios tested. At 6 h recovery, NK activity was still decreased significantly with the 12.5:1 and 3:1 E:T ratios. By 21 h recovery, NK activity did not differ significantly from baseline levels. Cortisol levels at 5 min post-exercise were negatively correlated with NK activity at 1.5 h recovery (r = -0.62, P = 0.05, 50:1 E:T ratio; r = -0.66, P = 0.04, 25:1 E:T ratio). Further research is needed to elucidate the effect these changes have on host immunosurveillance and immunoresponsiveness in vivo.
十名有经验的马拉松运动员在实验室中进行了3小时的运动。在0小时基线、1小时运动时以及恢复后的5分钟、1.5小时、6小时和21小时采集血样，分析表达自然杀伤（NK）细胞上膜抗原的淋巴细胞总数。同时也测量了NK活性。使用重复测量方差分析，所研究的七个淋巴细胞亚群中的四个，即Leu-11+19+、Leu-11+19-、Leu-11+7-和Leu-19+11-，随时间呈现出显著的受试者内效应。与基线值的简单对比显示，在恢复1.5小时和21小时时，携带三种不同NK标记组合（Leu-11+19+、Leu-11+19-和Leu-11+7-）的淋巴细胞总数与基线值相比显著减少。在恢复1.5小时时，所测试的六个效应性NK细胞/靶K562髓性白血病细胞（E:T）比例中的四个，NK活性显著低于基线水平。在恢复6小时时，12.5:1和3:1的E:T比例下，NK活性仍显著降低。到恢复21小时时，NK活性与基线水平无显著差异。运动后5分钟时的皮质醇水平与恢复1.5小时时的NK活性呈负相关（r = -0.62，P = 0.05，50:1 E:T比例；r = -0.66，P = 0.04，25:1 E:T比例）。需要进一步研究以阐明这些变化对体内宿主免疫监视和免疫反应性的影响。
