Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.
Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.
Anal Chim Acta. 2013 Apr 22;773:9-23. doi: 10.1016/j.aca.2012.12.040. Epub 2013 Jan 2.
Biosensors based on surface plasmon resonance (SPR) have become a central tool for the investigation and quantification of biomolecules and their interactions. Nucleic acids (NAs) play a vital role in numerous biological processes and therefore have been one of the major groups of biomolecules targeted by the SPR biosensors. This paper discusses the advances of NA SPR biosensor technology and reviews its applications both in the research of molecular interactions involving NAs (NA-NA, NA-protein, NA-small molecule), as well as for the field of bioanalytics in the areas of food safety, medical diagnosis and environmental monitoring.
基于表面等离子体共振（SPR）的生物传感器已成为研究和定量生物分子及其相互作用的重要工具。核酸（NAs）在许多生物过程中起着至关重要的作用，因此一直是 SPR 生物传感器的主要生物分子目标之一。本文讨论了 NA SPR 生物传感器技术的进展，并回顾了其在涉及 NAs 的分子相互作用研究（NA-NA、NA-蛋白质、NA-小分子）以及食品安全、医学诊断和环境监测等生物分析领域中的应用。
