Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Talanta. 2019 Jan 15;192:118-127. doi: 10.1016/j.talanta.2018.09.023. Epub 2018 Sep 11.
Surface plasmon resonance (SPR), as an optical technique, has widely been used for the detection of biomarkers. Various investigations have been conducted to address the impacts of SPR on the kinetics of biological interactions between the ligand and its cognate bio-element. Up until now, different biofunctionalized metal nanoparticles (NPs) have been used for the ultrasensitive detection of biomarkers in the enhanced SPR. The enhancement of plasmonic properties and refractive index by means of metal NPs in SPR-based biosensors have significantly improved the diagnosis and monitoring of molecular markers in different disesaes including malignancies. In all the enhanced SPR systems utilized for the direct/sandwich assay, each NP is covalently modified with the analyte molecules like antibody (Ab) or a nucleic acid such as DNA/RNA aptamer (Ap) capable of interaction with the related biomarker(s). The increasing of density near the gold surface and plasmonic coupling of gold film and NPs can provide a large shift in the refractive index enhancing the plasmonic resonance because the SPR response unit is sensitive to alteration of the refractive index and the mass shifting onto the chip surface. In this study, we review the potential applications of two major NPs for enhancing the SPR signals for the detection of molecular biomarkers, including gold and magnetic NPs.
表面等离子体共振（SPR）作为一种光学技术，已广泛应用于生物标志物的检测。已经进行了各种研究来解决 SPR 对配体与其同源生物元素之间生物相互作用动力学的影响。到目前为止，不同的生物功能化金属纳米粒子（NPs）已被用于增强 SPR 中生物标志物的超灵敏检测。SPR 生物传感器中金属 NPs 对等离子体特性和折射率的增强，显著改善了不同疾病（包括恶性肿瘤）中分子标志物的诊断和监测。在所有用于直接/三明治分析的增强 SPR 系统中，每个 NP 都通过与相关生物标志物（如抗体（Ab）或 DNA/RNA 适体（Ap）等核酸）相互作用的分析物分子（如抗体（Ab）或核酸（如 DNA/RNA 适体（Ap））进行共价修饰。金表面附近密度的增加和金膜与 NPs 的等离子体耦合可以提供折射率的大变化，从而增强等离子体共振，因为 SPR 响应单元对折射率的变化和质量转移到芯片表面非常敏感。在这项研究中，我们综述了两种主要的 NPs（金和磁性 NPs）增强 SPR 信号以检测分子生物标志物的潜在应用。
