Bioengineering College, Chongqing University, Chongqing 400044, PR China.
Int J Med Sci. 2013;10(6):691-8. doi: 10.7150/ijms.6170. Epub 2013 Apr 5.
Previous research has revealed that Wnt10b activates canonical Wnt signaling, which is integral to melanocyte differentiation in hair follicles (HFs). However, the function of Wnt10b in HF melanocytes remains poorly understood. We determined using Dct-LacZ transgenic mice that Wnt10b is mainly expressed near and within melanocytes of the hair bulbs during the anagen stage of the hair cycle. We also found that Wnt10b promotes an increase in melanocyte maturation and pigmentation in the hair bulbs of the mouse HF. To further explore the potential functions of Wnt10b in mouse HF melanocytes, we infected iMC23 cells with Ad-Wnt10b to overexpress Wnt10b. We demonstrated that Wnt10b promotes the differentiation of melanocytes by activating canonical Wnt signaling in melanocytes.
先前的研究表明，Wnt10b 激活了经典的 Wnt 信号通路，该通路对毛囊（HFs）中的黑素细胞分化至关重要。然而，Wnt10b 在 HF 黑素细胞中的功能仍知之甚少。我们使用 Dct-LacZ 转基因小鼠确定，在毛发生长周期的生长期，Wnt10b 主要在毛球内和毛球附近的黑素细胞中表达。我们还发现，Wnt10b 促进了小鼠 HF 毛球中黑素细胞的成熟和色素沉着增加。为了进一步探索 Wnt10b 在小鼠 HF 黑素细胞中的潜在功能，我们用 Ad-Wnt10b 感染 iMC23 细胞以过表达 Wnt10b。我们证明 Wnt10b 通过激活黑素细胞中的经典 Wnt 信号通路促进黑素细胞的分化。
