Patel Dk, Kumar R, Kumar M, Sairam K, Hemalatha S
Pharmacognosy Research Laboratory, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi-221005, India.
Asian Pac J Trop Biomed. 2012 Feb;2(2):134-9. doi: 10.1016/S2221-1691(11)60207-4.
To evaluate the aldose reductase inhibitory (ARI) activity of different fractions of Hybanthus enneaspermus for potential use in diabetic cataract.
Total phenol and flavonoid content of different fractions was determined. ARI activity of different fractions in rat lens was investigated in vitro.
The results showed significant level of phenolic and flavonoid content in ethyl acetate fraction [total phenol (212.15±0.79 mg/g), total flavonoid (39.11±2.27 mg/g)] and aqueous fraction [total phenol (140.62±0.57 mg/g), total flavonoid (26.07±1.49 mg/g)] as compared with the chloroform fraction [total phenol (68.56±0.51 mg/g), total flavonoid (13.41±0.82 mg/g)] and petrolium ether fraction [total phenol (36.68±0.43 mg/g), total flavonoid (11.55±1.06 mg/g)]. There was a significant difference in the ARI activity of each fraction, and it was found to be the highest in ethyl acetate fraction [IC50 (49.26±1.76 µg/mL)] followed by aqueous extract [IC50 (70.83±2.82 µg/mL)] and it was least in the petroleum ether fraction [IC50 (118.89±0.71 µg/mL)]. Chloroform fraction showed moderate activity [IC50 (98.52±1.80 µg/mL)].
Different fractions showed significanct amount of ARI activity, where in ethyl acetate fraction it was found to be maximum which may be due to its high phenolic and flavonoid content. The extract after further evaluation may be used in the treatment of diabetic cataract.
评估长萼鸡眼草不同提取物的醛糖还原酶抑制（ARI）活性，以探讨其在糖尿病性白内障治疗中的潜在应用价值。
测定不同提取物的总酚和黄酮含量。体外研究不同提取物对大鼠晶状体的ARI活性。
结果显示，与氯仿提取物[总酚（68.56±0.51mg/g），总黄酮（13.41±0.82mg/g）]和石油醚提取物[总酚（36.68±0.43mg/g），总黄酮（11.55±1.06mg/g）]相比，乙酸乙酯提取物[总酚（212.15±0.79mg/g），总黄酮（39.11±2.27mg/g）]和水提取物[总酚（140.62±0.57mg/g），总黄酮（26.07±1.49mg/g）]中的酚类和黄酮类含量显著更高。各提取物的ARI活性存在显著差异，其中乙酸乙酯提取物的活性最高[半数抑制浓度（IC50）为（49.26±1.76μg/mL）]，其次是水提取物[IC50为（70.83±2.82μg/mL）]，石油醚提取物的活性最低[IC50为（118.89±0.71μg/mL）]。氯仿提取物表现出中等活性[IC50为（98.52±1.80μg/mL）]。
不同提取物均表现出显著的ARI活性，其中乙酸乙酯提取物的活性最高，这可能与其较高的酚类和黄酮类含量有关。经过进一步评估后，该提取物可能用于糖尿病性白内障的治疗。
