Taraphdar Debjani, Sarkar Arindam, Chatterjee Shyamalendu
Indian Council of Medical Research Virus Unit, Kolkata.
Asian Pac J Trop Biomed. 2012 Feb;2(2):97-101. doi: 10.1016/S2221-1691(11)60200-1.
To develop a rapid, cost effective RT-PCR method for the mass scale diagnosis of such diseases at the viremia stage to find out the actual disease burden in that area.
For this purpose, cases with the history of only short febrile illness were considered. Thus 157 samples with the history of dengue/chikungunya like illness and only 58 samples with a history of acute encephalitis syndrome (AES) were selected.
Out of 157 samples, 42 and 74 were detected as dengue and chikungunya, respectively and out of 58 AES cases only 23 could be detected as Japanese encephalitis by this RT-PCR method.
This cost effective RT-PCR method can detect the total positive cases that remain undetected by ELISA method. Moreover, this method is capable to detect the viral RNA from patients' sera even after the appearance of IgM antibody at one fifth costs as compared with the other commercially available kits.
开发一种快速、经济高效的逆转录聚合酶链反应（RT-PCR）方法，用于在病毒血症阶段对这类疾病进行大规模诊断，以查明该地区的实际疾病负担。
为此，仅考虑有短期发热病史的病例。因此，选择了157例有登革热/基孔肯雅热样疾病病史的样本，以及仅58例有急性脑炎综合征（AES）病史的样本。
在157个样本中，分别检测出42例登革热和74例基孔肯雅热，在58例AES病例中，通过这种RT-PCR方法仅能检测出23例日本脑炎。
这种经济高效的RT-PCR方法可以检测出酶联免疫吸附测定（ELISA）方法未检测到的所有阳性病例。此外，与其他市售试剂盒相比，该方法能够以五分之一的成本，在患者血清中出现IgM抗体后仍检测到病毒RNA。
