Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Radiology, Massachusetts General Hospital, Boston, MA, USA.
Nanomedicine. 2013 Oct;9(7):1009-17. doi: 10.1016/j.nano.2013.03.011. Epub 2013 Apr 6.
Circulating tumor cells (CTC) harvested from peripheral blood have received significant interest as sources for serial sampling to gauge treatment efficacy. Nanotechnology and microfluidic based approaches are emerging to facilitate such analyses. While of considerable clinical importance, there is little information on how similar or different CTCs are from their shedding bulk tumors. In this clinical study, paired tumor fine needle aspirate and peripheral blood samples were obtained from cancer patients during image-guided biopsy. Using targeted magnetic nanoparticles and a point-of-care micro-NMR system, we compared selected biomarkers (EpCAM, EGFR, HER-2 and vimentin) in both CTC and fine needle biopsies of solid epithelial cancers. We show a weak correlation between each paired sample, suggesting that use of CTC as "liquid biopsies" and proxies to metastatic solid lesions could be misleading.
In this clinical study, paired tumor fine needle aspirate and peripheral blood samples were obtained from patients with solid epithelial cancers during image-guided biopsy. Using targeted magnetic nanoparticles and a point-of-care micro-NMR system, the authors compared selected biomarkers in both circulating tumor cells (CTC) and fine needle biopsies, demonstrating a weak correlation between each paired sample, suggesting that use of CTC could be misleading in this context.
循环肿瘤细胞（CTC）从外周血中采集，作为连续采样的来源，以评估治疗效果，受到了极大的关注。基于纳米技术和微流控的方法正在涌现，以促进此类分析。虽然具有重要的临床意义，但关于 CTC 与其脱落的实体肿瘤之间的相似性或差异性的信息很少。在这项临床研究中，在影像学引导活检期间，从癌症患者中获得了配对的肿瘤细针抽吸和外周血样本。使用靶向磁性纳米颗粒和即时护理微 NMR 系统，我们比较了实体上皮癌的 CTC 和细针活检中选定的生物标志物（EpCAM、EGFR、HER-2 和波形蛋白）。我们显示出每个配对样本之间的相关性较弱，这表明将 CTC 用作“液体活检”和转移性实体病变的替代物可能会产生误导。
在这项临床研究中，在影像学引导活检期间，从患有实体上皮癌的患者中获得了配对的肿瘤细针抽吸和外周血样本。使用靶向磁性纳米颗粒和即时护理微 NMR 系统，作者比较了循环肿瘤细胞（CTC）和细针活检中选定的生物标志物，显示每个配对样本之间的相关性较弱，表明在这种情况下，使用 CTC 可能会产生误导。
