Freiburg Materials Research Center and Institute for Macromolecular Chemistry, Albert-Ludwigs-Universitaet Freiburg, Stefan-Meier-Str. 21, 79104 Freiburg, Germany.
J Biomater Sci Polym Ed. 2013;24(13):1589-600. doi: 10.1080/09205063.2013.782803. Epub 2013 Apr 10.
The goal of this study was to develop a long-term active antimicrobial coating for surgical sutures. To this end, two water-insoluble polymeric nanocontainers based on hyperbranched polylysine (HPL), hydrophobically modified by either using glycidyl hexadecyl ether, or a mixture of stearoyl/palmitoyl chloride, were synthesized. Highly stabilized silver nanoparticles (AgNPs, 2-5 nm in size) were generated by dissolving silver nitrate in the modified HPL solutions in toluene followed by reduction with L-ascorbic acid. Poly(glycolic acid)-based surgical sutures were dip-coated with the two different polymeric silver nanocomposites. The coated sutures showed high efficacies of more than 99.5% reduction of adhesion of living Staphylococcus aureus cells onto the surface compared to the uncoated specimen. Silver release experiments were performed on the HPL-AgNP modified sutures by washing them in phosphate buffered saline for a period of 30 days. These coatings showed a constant release of silver ions over more than 30 days. After this period of washing, the sutures retained their high efficacies against bacterial adhesion. Cytotoxicity tests using L929 mouse fibroblast cells showed that the materials are basically non-cytotoxic.
本研究旨在开发一种用于外科缝线的长效主动抗菌涂层。为此，合成了两种基于支化多赖氨酸（HPL）的水不溶性聚合物纳米容器，通过使用缩水甘油十六醚或硬脂酰/棕榈酰氯的混合物对其进行疏水改性。将硝酸银溶解在改性 HPL 的甲苯溶液中，然后用 L-抗坏血酸还原，生成高度稳定的银纳米颗粒（AgNPs，大小为 2-5nm）。用两种不同的聚合物银纳米复合材料对聚（乙醇酸）基外科缝线进行浸涂。与未涂层的样品相比，涂层缝线对活金黄色葡萄球菌细胞在表面的粘附具有超过 99.5%的高效抑制作用。通过在磷酸盐缓冲盐水中洗涤缝线 30 天，对 HPL-AgNP 改性缝线进行了银释放实验。这些涂层在 30 多天内持续释放银离子。经过这段洗涤期后，缝线仍能保持对细菌粘附的高效抑制作用。使用 L929 小鼠成纤维细胞的细胞毒性测试表明，这些材料基本无细胞毒性。
