School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
Virol Sin. 2013 Apr;28(2):103-8. doi: 10.1007/s12250-013-3308-7. Epub 2013 Apr 11.
Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China.
人腺病毒（HAdV），特别是 HAdV-B3、-E4 和 -B7，与中国儿童的急性呼吸道疾病有关，偶尔也会在成人中出现。为了确定和记录中国南方广州地区儿童呼吸道腺病毒病原体的特征，建立了一种快速、简单和实用的呼吸道腺病毒鉴定和分型方法，并对其进行了评估。根据六邻体基因保守区设计了一对通用 PCR 引物，该引物不仅可以检测 HAdV-B3、-E4 和 -B7，还可以检测 HAdV-B14、-F40 和 -F41，具有特定的 300bpPCR 产物。根据六邻体基因的高变区还设计了三对型特异性 PCR 引物，通过三个独立的 PCR 反应来分型 HAdV-B3、-E4 和 -B7，这使得优化 PCR 条件变得容易。应用该方法对 2010 年 10 月至 2011 年 12 月期间采集的 100 份疑似腺病毒感染的咽拭子标本进行了鉴定和分型。这些样本中，有 55 份为腺病毒阳性。最常见的 HAdV 型是 HAdV-B3，在 92.7%的样本中被鉴定为阳性，这不仅与 2004-2006 年报道的数据一致，也与最近中国东部杭州的报道一致，表明 HAdV-B3 已在中国广州流行多年，可能也在中国东部流行多年。我们建立的呼吸道腺病毒鉴定和分型方法快速、简单、实用，对于实时监测流行腺病毒株具有潜在应用价值，也为中国腺病毒相关疾病的控制和预防提供了病因学证据。
